山东医药2017，Vol.57Issue(21)：9-12,4.DOI:10.3969/j.issn.1002-266X.2017.21.003
HULC在人脑胶质瘤组织中表达变化及对肿瘤细胞增殖侵袭的调控机制
Expression of HULC in human glioma and its regulatory mechanism on proliferation and invasion of tumor cells
摘要
Abstract
Objective To observe the expression of highly up-regulated in liver cancer (HULC) in human glioma and to explore its regulatory mechanism on proliferation and invasion of glioma cells. Methods Nineteen cases of normal brain tissues, 13 cases of low-grade, and 39 cases of high-grade glioma tissue samples were collected, and the expression of HULC was detected by RT-PCR. Human glioma cells U87 were randomly divided into the infection group 1, infection group 2, and control group. In vitro, U87siHULC 1 and U87siHULC 2 constructed with lentiviral transfection were used to interfere the expression of HULC in the infection group 1 and infection group 2, respectively. RT-PCR was used to detect interference efficiency. MTT was used to detect the ability of cell proliferation (490 nm relative absorbance), and Transwell invasion assay was used to detect the ability of the invasion (transmembrane cell number). Western blotting was used to detect the protein expression of epithelial mesenchymal transition (EMT)-related molecules including E-cadherin, Snail and Vimentin, and cancer stemness-related molecule CD133 and invasion-related molecule matrix metalloproteinase 2 (MMP-2). Results The expression level of HULC mRNA in high-grade gliomas was higher than that in low-grade gliomas and normal brain tissues (all P<0.05). The expression level of HULC mRNA, the ability of cell proliferation and invasion, transmembrane cell number, and the expression of E-cadherin, Snail, Vimentin, MMP-2, and CD133 of the infection group 1 and infection group 2 were higher than those of the control group (all P<0.05). Conclusion HULC is highly expressed in human glioma tissues, and can promote cell proliferation and invasion by promoting the process of cell EMT and up-regulating the expression of MMP-2 and CD133.关键词
脑胶质瘤/长链非编码RNA/肝癌高表达转录本/细胞增殖/细胞侵袭Key words
glioma/long non-coding RNA/highly up-regulated in liver cancer/cell proliferation/cell invasion分类
医药卫生引用本文复制引用
刘达远,张彩彩..HULC在人脑胶质瘤组织中表达变化及对肿瘤细胞增殖侵袭的调控机制[J].山东医药,2017,57(21):9-12,4.基金项目
海南省自然科学基金资助项目(20158308). （20158308）
