山东医药2017，Vol.57Issue(21)：17-20,4.DOI:10.3969/j.issn.1002-266X.2017.21.005
肾细胞癌组织中RASSF10基因表达及其启动子区甲基化变化的意义
Expression of RASSF10 gene in renal cell carcinoma and its promoter methylation
摘要
Abstract
Objective To observe the expression of RASSF10 gene in renal cell carcinoma (RCC) and its promoter methylation, and to investigate its significance. Methods The mRNA expression and methylation rate of RASSF10 gene in RCC tissues, para-carcinoma tissues, and normal tissues of 52 cases were detected by using reverse transcription polymerase chain reaction (RT-PCR) and methylation-specific polymerase chain reaction (MSP). Their relationship with pathological features of RCC patients and the relationship between mRNA expression and methylation of RASSF10 gene were analyzed. Results The mRNA relative expression level of RASSF10 gene in RCC tissues was lower, and the methylation rate was higher than that in the para-carcinoma tissues and normal tissues (both P<0.05). The mRNA expression and promoter methylation rate of RASSF10 gene in RCC tissues were not correlated with age, histological type, tumor size, and Fuhrman grade (all P>0.05), but were correlated with tumor TNM grade (P<0.05). The mRNA relative expression of RASSF10 gene in RCC patients with methylation was lower than that without methylation (P<0.05).Conclusion The low expression of RASSF10 gene in RCC tissues may be related to promoter hypermethylation, and low expression of RASSF10 gene and its promoter methylation may participate in the occurrence and development of RCC.关键词
肾细胞癌/RASSF10基因/基因启动子区甲基化Key words
renal cell carcinoma/RASSF10 gene/gene promoter methylation分类
医药卫生引用本文复制引用
樊博,杨俊,张爱莉,赵志红,倪晓辰,刘彬,马永良,任宗涛..肾细胞癌组织中RASSF10基因表达及其启动子区甲基化变化的意义[J].山东医药,2017,57(21):17-20,4.基金项目
河北省医学科学研究重点课题(20110142). （20110142）
