实用医学杂志2017，Vol.33Issue(7)：1021-1024,4.DOI:10.3969/j.issn.1006-5725.2017.07.001
神经生长因子对脑梗死大鼠VEGF、SDF-1的影响及其可能机制
Effects and possible mechanisms of nerve growth factor on serum levels of VEGF and SDF-1 protein expression in rats with focal cerebral ischemia
摘要
Abstract
Objective To investigate the effects and the possible mechanisms of nerve growth factor (NGF) on serum levels of vascular endothelial growth factor (VEGF) and stromal cell-derived factor 1 (SDF-1) protein expression in rats with focal cerebral ischemia.Methods Male Sprague-Dawley rats weighting 200 ～ 250 g were subjected to middle cerebral occlusion (MCAO).MCAO rats were randomly divided into NGF group,saline control group and NGF+PI3K antagonist group (n =12).The neurological function was evaluated on the 4th,7th day after MCAO,and the serum levels of VEGF and SDF-1 protein were measured by ELISA.Results The neurological function was better in rats of the NGF group than those of the saline control group and NGF+PI3K antagonist on the 4th,7th day after MCAO (P ＜ 0.05).The serum levels of VEGF and SDF-1 protein of the NGF group was significantly higher than that of the saline control group and NGF+PI3K antagonist group on the 4th day after MCAO (P ＜ 0.05).Conclusions Treatment with NGF may improve neurological function of rats with focal cerebral ischemia,and upregulate serum levels of VEGF and SDF-1 protein expression.PI3K/AKT signal pathway may have attended the above regulation.关键词
脑梗死/神经生长因子/大脑中动脉梗死/血管内皮生长因子/基质细胞衍生因子-1Key words
Cerebral infarction/Nerve growth factor/Middle cerebral artery occlusion/VEGF/SDF-1引用本文复制引用
李小强,凌莉,李楚翘,马文斌,尹义臣..神经生长因子对脑梗死大鼠VEGF、SDF-1的影响及其可能机制[J].实用医学杂志,2017,33(7):1021-1024,4.基金项目
国家自然科学基金项目(编号:81100898) （编号:81100898）
广东省自然科学基金项目(编号:2014A030313681) （编号:2014A030313681）
