天然产物研究与开发2017，Vol.29Issue(5)：735-740,782,7.DOI:10.16333/j.1001-6880.2017.5.003
细辛苯丙素类化合物生物合成相关酶基因的挖掘及其表达分析
Mining and Expression Analysis of Enzyme Genes Relevant to the Biosynthesis of Phenylpropanoids in Asarum
摘要
Abstract
In order to explore the expression level of enzyme genes relevant to the biosynthesis of phenylpropanoids in Asarum,the enzyme genes relevant to the biosynthesis of phenylpropanoids were screened out according to the gene functional annotation which was based on the database of Asarum transcriptome constructed in the laboratory.The protein sequence was deduced and was analyzed by bioinformatics.Meanwhile,expression analysis of the enzyme genes was carried out.Six enzyme genes were screened out from the transcriptome of Asarum,including pal,c4h,comt,4cl,ccr and cad.The protein sequence encoded by six enzyme genes had different superfamily.The expression level of six enzyme genes showed the same changes.For leaf,petiole,root and rhizome,the expression level of six enzyme genes in flowering stage was higher than that in early flowering stage,and the expression level of six enzyme genes in the underground part was higher than that in the aboveground part.The above results showed thatthe expression level of six enzyme genes in leave,petiole,root and rhizome of Asarum was different,but there was a certain regularity.The success of this study will lay a foundation for further understanding of the functions and characteristics of the enzymesrelevant to the biosynthesis of phenylpropanoids,and provide a theoretical basis for future biosynthesis and metabolic regulation of active ingredients in Asarum.关键词
细辛/苯丙素/甲基丁香酚/榄香素/黄樟醚/生物信息学分析/实时荧光定量PCRKey words
Asarum/phenylpropanoid/methyl eugenol/elemene/safrole/bioinformatics analysis/quantitative real-time PCR分类
医药卫生引用本文复制引用
潘磊,林懋怡,赵晓冰,柳威,刘晋杰,刘忠..细辛苯丙素类化合物生物合成相关酶基因的挖掘及其表达分析[J].天然产物研究与开发,2017,29(5):735-740,782,7.基金项目
国家自然科学基金(81373962) （81373962）
上海市自然科学基金(13ZR1420900) （13ZR1420900）
