天然产物研究与开发2017，Vol.29Issue(5)：767-773,7.DOI:10.16333/j.1001-6880.2017.5.008
大蓟炭中香叶木素诱导人乳腺癌MCF-7细胞凋亡及其机制研究
Effects of Diosmetin from Carbonized Cirsium japonicum on Apoptosis in Human MCF-7 Breast Cancer Cells and its Mechanisms
摘要
Abstract
To study the effects of diosmetin from carbonized Cirsium japonicum on apoptosis in human MCF-7 breast cancer cells and its mechanisms.Three flavonoids were isolated and purified from carbonized C.japonicum by silica gel and Sephadex LH-20 chromatography methods.Their structures were elucidated by NMR and MS spectroscopic data and identified as diosmetin(1),acacetin (2) and pectolinarigenin (3).MTS assay was performed to detect the viability of MCF-7 cells treated by different concentrations of diosmetin.The cell apoptosis rate was further analyzed by flow cytometry (FCM).Western blot assay was applied to measure the apoptosis related protein expression levels of PARP,P-JNK.Diosmetin treatment on MCF-7 cells significantly inhibited cell proliferation and induced cell apoptosis.Diosmetin significantly downregulated P-JNK and upregulated cleaved-PARP protein expression.Diosmetin inhibited MCF-7 cell proliferation and induced cell apoptosis by activation of JNK pathway in vitro.关键词
大蓟炭/香叶木素/人乳腺癌MCF-7细胞/细胞凋亡/作用机制Key words
diosmetin/human breast cancer cell line MCF-7/cell apoptosis/mechanism分类
医药卫生引用本文复制引用
姚亮亮,王晓珊,何军伟,岳丹洁,蒋福全,郑里翔..大蓟炭中香叶木素诱导人乳腺癌MCF-7细胞凋亡及其机制研究[J].天然产物研究与开发,2017,29(5):767-773,7.基金项目
江西中医药大学校级科研项目(2013ZR004) （2013ZR004）
国家自然科学基金(81160531) （81160531）
江西省自然科学基金(2016BAB205223) （2016BAB205223）
