天然产物研究与开发2017，Vol.29Issue(5)：831-835,786,6.DOI:10.16333/j.1001-6880.2017.5.020
白藜芦醇对大鼠肾脏缺血再灌注损伤的保护作用
Protective Effect of Resveratrol on Renal Ischemia Reperfusion Injury of Rats
摘要
Abstract
To explore the underlying protection and mechanism of RSV in renal ischemia reperfusion injury (IRI).The model of IRI was induced by clamping the left renal pedicles with right nephrectomy.SD rats were randomly divided into Sham group(Sham),Renal ischemia reperfusion injury group (IRI),and Resveratrol pretreatment group (RSV).The morphology of kidney was exanimed by HE;The expression of Cr and BUN were measured by ELISA;TUNEL was used to examine the cell apoptosis;The expression of SIRT1,p53,Acetylp53,Bax,BCL-2,PUMA-α and cytochrome c were evaluated by Western blot analysis.The results showed RSV pretreatment can significantly decrease the expression of TUNEL,BUN,Cr,Acetyl 53,PUMA-α and Bax by increaseing the expression of SIRT1 and BCL-2.Moreover,RSV pretreatment can also attenuate the change of morphology of kidney and the transcolation of cytochrome c,which indicated RSV pretreatment can protect kidney against ischemia reperfusion injury by suppressing apoptosis.The mechanism was associated with attenuating Acetylp53 by activating SIRT1.关键词
白藜芦醇/肾脏缺血再灌注损伤/凋亡/SIRT1/p53Key words
resveratrol/renal ischemia reperfusion injury/apoptosis/SIRT1/p53分类
医药卫生引用本文复制引用
王聪,王程..白藜芦醇对大鼠肾脏缺血再灌注损伤的保护作用[J].天然产物研究与开发,2017,29(5):831-835,786,6.基金项目
国家自然科学基金(81500575) （81500575）
