天然产物研究与开发2017，Vol.29Issue(5)：836-842,7.DOI:10.16333/j.1001-6880.2017.5.021
光甘草定诱导小鼠黑色素瘤B16F10细胞凋亡的机制研究
Glabridin Induces Apoptosis in Melanoma B16F10 Cells by Altering Glycolysis
摘要
Abstract
To explore the molecular mechanism of glabridin-induced B16F10 cell proliferation inhibition via in vitro and in vivo model of melanoma.Glabridin induced B16F10 cell proliferation inhibition and cell apoptosis in a dose-dependent manner.The Bax mRNA and protein expressions were up-regulated and the Bcl-2 was down-regulated in glabridin-treated B16F10 cells.Further studies showed that glabridin decreased the production of ATP and lactic acid in mouse melanoma B16F10 cells.Glabridin down-regulated the mRNA and protein expressions of glycolysis-related kinase genes (Hk2 and Ldha) in B16F10 cells.In addition,the animal model of mouse melanoma indicated that glabridin treatment suppressed tumor growth and induced tumor apoptosis.Both the mRNA and protein levels of glycoIysis-related kinase genes(Hk2 and Ldha) and Bcl-2 in tumor tissue were also decreased and Bax was increased in glabridin treated mice.It was concluded thatglabridin inhibited the proliferation and induced apoptosis of melanoma B16F10 cells in a dose-dependent manner,and the inhibition of glycolysis played a crucial role in the induction of apoptosis in glabridin-treated B16F10 cells.关键词
光甘草定/B16F10细胞/糖酵解/细胞凋亡Key words
glabridin/B16F10 cells/glycolysis/apoptosis分类
医药卫生引用本文复制引用
高彩霞,王艳明,李德芳,唐晓峰,郑秋生..光甘草定诱导小鼠黑色素瘤B16F10细胞凋亡的机制研究[J].天然产物研究与开发,2017,29(5):836-842,7.基金项目
滨州医学院科研启动基金(BY2014KYQD01) （BY2014KYQD01）
国家自然科学基金(31471338) （31471338）
山东省高等学校优势学科人才团队培育计划 （）
