天然产物研究与开发2017，Vol.29Issue(5)：862-866,820,6.DOI:10.16333/j.1001-6880.2017.5.025
丹酚酸A的分离纯化工艺及其抗氧化活性的研究
Separation, Purification and Antioxidant Activity of Salvianolic Acid A from Salvia miltiorrhiza var.alba
摘要
Abstract
To optimize the separation and purification technology of salvianolic acid A from Salvia miltiorrhiza var.alba with polymide resin.The antioxidant activity of the purified salvianolic acid A were studied in vitro.With adsorption capacity and desorption rate as index,static and dynamic adsorption-desorption were used to determine the optimal separation and purification conditions of salvianolic acid A.The optimal conditions of polymide resin were as follows:sample injection concentration was 11 g/L,sample flow rate was 1.0 mL/min,quantity of adsorption was 150 mL,desorption rate was 1.0 mL/min,and elution volume was 10 BV of 50％ ethanol.Mter the purification,the purity of obtained salvianolic acid A was 40.36％.Moreover,the antioxidant activity of the extract were evaluated by DPPH scavenging assay and reducing power.The results showed that the obtained salvianolic acid A had good antioxidant activity.关键词
白花丹参/丹酚酸A/聚酰胺树脂/工艺优化/抗氧化活性Key words
Salvia miltiorrhiza var.alba/salvianolic acid A/polymide resin/optimal process/antioxidant activity分类
医药卫生引用本文复制引用
张愉,季梅,孙隆儒..丹酚酸A的分离纯化工艺及其抗氧化活性的研究[J].天然产物研究与开发,2017,29(5):862-866,820,6.基金项目
国家自然科学基金(81274031) （81274031）
山东省科技发展计划(2014GSF119026) （2014GSF119026）
