土壤与作物2017，Vol.6Issue(2)：146-152,7.DOI:10.11689/j.issn.2095-2961.2017.02.010
利用CRISPR/Cas9创造早熟香味水稻
Breeding of Early-maturatity and Fragrant Rice via CRISPR/Cas9 Mediated Genome Editing
摘要
Abstract
Heilongjiang province is the largest rice cultivation region,and the growth area increased dramatically over the past years.To develop the variety with both early-maturatity and high quality,which can be grown in higher latitude region with lower temperature,in this study,through CRISPR/Cas9 mediated genome editing,we knocked out the flowering time gene Hd2 and fragrance gene Badh2 in Suijing14 via,a major elite cultivar in the second accumulated temperature zone.Two edited lines were generated and defined as Suijing14-d1 and Suijing14-d2.At T1 generation,through molecular characterization,representative Suijing14-d1 and Suijing14-d2 seedlings were chosen in which both Hd2 and Badh2 were mutated and are homozygous,and the Hygromycin resistance gene was omitted..Field statistical analysis demonstrated that the heading date of Suijing14-d1 and Suijing14-d2 was shortened around 13days compared with Suijing14.Seeds aromas were preliminarily analyzed by chewing and potassium hydroxide extraction assay.Gas Chromatography-Mass Spectrometry (GC-MS) assay was further performed to quantify the aroma compound,2-acetyl-1-pyrrolin (2-AP) in mature seeds,and it was found that the 2-AP content in Suijing14-d1 and Suijing14-d2 was significantly higher than that in Suijing14.In summary,we successfully generated early-maturatity and fragrant rice variety via CRISPR/Cas9 mediated genome editing,which will markedly facilitate the local breeding program.关键词
水稻/香味/Badh2/抽穗期/Hd2/CRISPR/Cas9Key words
rice/fragrance/Badh2/heading date/Hd2/CRISPR/Cas9分类
农业科技引用本文复制引用
周文甲,田晓杰,任月坤,魏祥进,高扬,谢黎虹,刘华招,卜庆云,李秀峰..利用CRISPR/Cas9创造早熟香味水稻[J].土壤与作物,2017,6(2):146-152,7.基金项目
黑龙江省重点基金(ZD2015005) （ZD2015005）
哈尔滨市优秀人才项目(RC2014XK002003). （RC2014XK002003）
