西南林业大学学报2017，Vol.37Issue(3)：14-20,7.DOI:10.11929/j.issn.2095-1914.2017.03.003
速生型杉木SRAP遗传差异分析
Genetic Divergence of the Chinese Fir Fast-growing Genotypes Revealed by SRAP Markers
摘要
Abstract
In this study, sequence-related amplified polymorphism (SRAP) markers were used to genotype 32 fast-growing Chinese fir ( Cunninghamia lanceolata) clones, and therefore evaluated the polymorphisms and genetic similarity, and generated a UPGMA dendrogram of the clones. The result indicated that 21 SRAP primer combina-tions produced 237 clear and stable bands, and notably, 214 of these were polymorphic. Different primer combina-tions seemed to have a divergent discrimination power in clones with NG ( number of genotype identified) of 10-31. Based on the SRAP markers, the population diversity parameters of PPB ( percentage of polymorphism band) , PIC ( polymorphism information content) , GD ( gene diversity) , Na ( number of different alleles) , Ne ( number of ef-fective alleles), I (Shannon′s information index) and He (expected heterozygosity) were found to be 90. 3%, 0. 2187, 0. 2682, 1. 903、 1. 392、 0. 380 and 0. 243 respectively, reflecting a moderate degree of diversity in present Chinese fir collection. Impressively, the SRAP-based UPGMA tree help clarify the molecular genetic dis-tance of the clones with a pairwise genetic similarity coefficient of 0. 4000-0. 5999 for most ( 95. 5%) of the clone pairs. Briefly, it could be concluded that there still has large amount of variation among clones ( n=32) , albeit a restricted selection for their growth traits ( fast-growing) . Intra-population ( n=32) reselection and crossing is rather promising for this collection.关键词
杉木/分子标记/遗传多态性/聚类分析Key words
Chinese fir/molecular marker/genetic polymorphism/cluster analysis分类
农业科技引用本文复制引用
郑会全,胡德活,王润辉,吴淑娟..速生型杉木SRAP遗传差异分析[J].西南林业大学学报,2017,37(3):14-20,7.基金项目
林业公益性行业科研专项经费 (201404127) 资助 （201404127）
"广东特支计划" 科技创新青年拔尖人才项目 (2014TQ01N140) 资助. （2014TQ01N140）
