西南林业大学学报2017，Vol.37Issue(3)：21-25,5.DOI:10.11929/j.issn.2095-1914.2017.03.004
4个西南桦品系的遗传多样性分析
Genetic Diversity of 4 Betula alnoides
摘要
Abstract
Four Betula alnoides strains in Yunnan Province were selected and the total genomic DNA was ex-tracted with the modified CTAB method, by contrast with commercial strain, the relations of 4 B. alnoides strains′s genetic diversity, genetic distance and genetic clustering were studied. The result indicated that 4 pairs of specific primers have been screen out from 121 pair of primers by SSR molecular marker technique, and used for SSR-PCR amplification. 60 sites were amplified, and the percentage of polymorphic sites PPB=95. 00%, Nei′s gene diversity index H=0. 3265, Shannon information index I=0. 4897, genetic differentiation coefficient between population Gst=0. 2114. The genetic diversity of all the B. alnoides samples was high, and the genetic variation was mainly in the strain. The genetic distance between strain1 and strain2 was the closest ( D=0. 0891) , strain3 and strain4 has the farthest ( D=0. 1727) . The cluster analysis of UPGAM showed that there were 3 categories, strain1 and strain2 were in one category, strain4 and commercial strain were in the second category, and strain3 in the third category.关键词
西南桦/SSR/品系/遗传多样性Key words
Betula alnoides/SSR/strain/genetic diversity分类
农业科技引用本文复制引用
睢鑫洲,郑毅,仇玉萍,聂艳丽..4个西南桦品系的遗传多样性分析[J].西南林业大学学报,2017,37(3):21-25,5.基金项目
云南省科技计划重点新产品项目 (2014BB010) 资助 （2014BB010）
云南省技术创新人才项目 (2016HB017) 资助. （2016HB017）
