西南林业大学学报2017，Vol.37Issue(3)：32-39,8.DOI:10.11929/j.issn.2095-1914.2017.03.006
小木漆组织培养技术研究
Tissue Culture of Toxicodendron vernicifluum
摘要
Abstract
The stem segments from elite Toxicodendron vernicifluum tree were used as explants to establish the technical system of tissue culture. The disinfection and browning protection of stem segments, the medium of axillary bud startup, adventitious buds proliferation and seedlings rooting, and the conditions of plantlet transplan-ting were studied. It was found that the best disinfection method of explants was to be successively soaked in 75%ethanol for 20 s, 0. 05% benzalkonium for 1 min, and 0. 1% mercuric chloride for 20 min, and the explants was pollution-free. White medium supplemented with 100 mg/L cysteine could reach the lowest browning rate ( 1. 15%) . The optimum medium for axillary bud startup was White+100 mg/L cysteine+2. 0 mg/L 6-BA+0. 5 mg/L NAA, with a germination rate of 91. 67%. The optimum medium for seedling proliferation was MS+0. 5 mg/L ZT+1. 0 mg/L 6-BA+0. 1 mg/L TDZ, with a multiplication coefficient over 4. 89. Culture medium for seedlings rooting was 1/2 MS+0. 05 mg/L IAA+0. 2 mg/L IBA, with a rooting rate of 98. 89% and an average root number per plantlet of 3. 98. The survival rate of complete plantlets was more than 80% when transplanted into the medium made up 50% humus and 50% river sand.关键词
小木漆/茎段/组织培养/植株再生Key words
Toxicodendron vernicifluum/stem section/tissue culture/plantlet regeneration分类
农业科技引用本文复制引用
罗一然,韩国伟,张雪,李旦,刘树娜,赵月明,和润喜,何承忠..小木漆组织培养技术研究[J].西南林业大学学报,2017,37(3):32-39,8.基金项目
云南省教育厅重大专项项目 (ZD2013014) 资助 （ZD2013014）
云南省教育厅研究生基金项目 (2016YJS100) 资助 （2016YJS100）
云南省林学一流学科建设项目 (51600625) 资助 （51600625）
西南林业大学大学生创新基金项目 (C16011, C16013) 资助. （C16011, C16013）
