新医学2017，Vol.48Issue(6)：380-384,5.DOI:10.3969/j.issn.0253-9802.2017.06.004
白芍总苷治疗大鼠急性痛风性关节炎的作用及对血清IL-1β、IL-6、TNF-α的影响
Effect of total glucosides of peony therapy on acute gouty arthritis and serum levels of IL-1β,IL-6 and TNF-α in rat models
摘要
Abstract
Objective To evaluate the effect of total glucosides of peony upon acute gouty arthritis (AGA) and serum levels of IL-6,IL-1β and TNF-α in AGA rat models.Methods Forty-eight male SD rats were chosen and randomly divided into the control,model,colchicine (0.8 mg/kg),low,moderate and high dose (75,150 and 300 mg/kg) of total glucosides of peony groups (n=8 for each group).At 5 d after medicine administration,AGA rat models were established by injection of microcrystalline monosodium urate (MSU) suspension in the ankle joint.The diameter of ankle joint at baseline and different time points after model establishment was measured.The difference in the ankle joint diameter before and after model establishment was used to reflect the degree of knee joint swelling.After experiment,blood sampling was collected via the abdominal aorta.The serum levels of IL-6,IL-1β and TNF-α were quantitatively measured by using ELISA and statistically compared among different groups.Results Compared with the control group,the degree of knee joint swelling in the model group was significantly enhanced at different time points (all P<0.01).Compared with the model group,the degree of joint swelling in the low-,moderate-and high-dose total glucosides of peony,and colchicine groups was significantly decreased at different time points (all P<0.05).No statistical significance was identified among different treatment groups (all P>0.05).Compared with the control group,the serum levels of IL-6,IL-1β and TNF-α in the model group were significantly up-regulated(all P<0.01).At 24 h after model establishment,the serum levels of IL-6,IL-1β and TNF-α in the low-,moderate-and high-dose total glucosides of peony and colchicine groups were considerably down-regulated than those in the model group (all P<0.01).No statistical significance was documented among different treatment groups (all P>0.05).Conclusion Use of total glucosides of peony exerts therapeutic effect upon AGA,probably by suppressing the production of pro-inflammatory cytokines of IL-6,IL-1β and TNF-α induced by MSU.关键词
白芍总苷/急性痛风性关节炎/肿瘤坏死因子-α/白介素-1β/白介素-6/大鼠Key words
Total glucosides of peony/Acute gouty arthritis/Tumor necrosis factor-α/Interleukin-1β/Interleukin-6/Rat引用本文复制引用
李振彬,马旭,杨静,刘彦卿,路占忠,李达,刘乐,刘芝..白芍总苷治疗大鼠急性痛风性关节炎的作用及对血清IL-1β、IL-6、TNF-α的影响[J].新医学,2017,48(6):380-384,5.基金项目
河北省科技计划项目(13277704D) （13277704D）
河北省中医药管理局科研计划课题(2015180) （2015180）
