针刺研究2017，Vol.42Issue(3)：189-196,8.DOI:10.13702/j.1000-0607.2017.03.001
耳穴经皮电刺激对颞叶癫痫大鼠癫痫发作频率与海马区胶质细胞活性及炎性因子的影响
Transcutaneous Electrostimulation of Auricular Otopoints Reduces Epileptic Attack Possibly by Suppressing Hippocampal Gliocyte Proliferation and Regulating IL-6 and IL-10 Expression in Chronic Temporal Lobe Epilepsy Rats
摘要
Abstract
Objective To observe the effect of transcutaneous otopoint electrostimulaiton (TCOES) on seizure frequency,immunoreactivity of hippocampal gliocytes and expression of proinflammatory cytokine interleukin-6(IL-6) and anti-inflammatory cytokine IL-10 in chronic temporal lobe epilepsy (CTLE) rats,so as to investigate its antiepileptic mechanism.Methods Thirty-six SD rats were randomly divided into control,model and TCOES groups (n=12 in each group).The CTLE model was established by intraperitoneal injection (i.p.i.) of lithium chloride (127.2 mg/kg),scopolamine (1 mg/kg,20 h after the 1st injection) and pilocarpine (10 mg/kg,30 min after scopolamine injection).Rats of the control group were treated by i.p.i.of normal saline.TCOES (1 mA,20 Hz) was applied to bilateral otopoint "Heart"-"Lung"-"Subcortex" region for 20 min,once daily for 6 weeks.The epileptic attack was observed by a video monitoring system.The numbers of ionized calcium-binding adapter molecule-1(Iba 1)-labeled microgliacytes and glial fibrillary acidic protein (GFAP)-labeled astrocytes in the CA 1 and CA 3 regions of hippocampus were counted under light microscope after immunostaining,and the expression levels of hippocampal IL-6 and IL-10 proteins and genes were determined by immunofluorescence and quantitative real-time PCR,respectively.Results After TCOES intervention,the seizure frequency was significantly decreased in comparison with pre-treatment(P<0.05),modeling-induced dramatic increase of the numbers of microgliacytes and astrocytes,IL-6 immunoactivity in the hippocampal CA 1 and CA 3 regions,and IL-6 mRNA expression in the hippocampus were significantly suppressed (P<0.05),and hippo-campal IL-10 immunoactivity and mRNA expression were considerably up-regulated in comparison with the model group (P<0.05).Conclusion TCOES intervention has an antiepileptic effect in CTLE rats,which may be associated with its effects in suppressing gliocyte proliferation,suppressing the expression of proinflammatory cytokine IL-6,and up-regulaiting the expression of anti-inflammatory cytokine IL-10 in the hippocampus.关键词
颞叶癫痫/经皮耳穴电刺激/海马/胶质细胞/炎性因子Key words
Chronic temporary lobe epilepsy/Transcutaneous otopoint electrical stimulation/Hippocampus/Glial cells/Inflammatory factors分类
医药卫生引用本文复制引用
乔丽娜,杨海龙,谭连红,杨娇娇,高巧玲,周胜红,张永臣,杨永升..耳穴经皮电刺激对颞叶癫痫大鼠癫痫发作频率与海马区胶质细胞活性及炎性因子的影响[J].针刺研究,2017,42(3):189-196,8.基金项目
国家自然科学基金面上项目(No.81273833) （No.81273833）
国家自然科学基金青年基金项目(No.81303055) （No.81303055）
国家重点基础研究发展计划"973"项目(No.2014CB543103、2013CB531904) （No.2014CB543103、2013CB531904）
