针刺研究2017，Vol.42Issue(3)：202-208,216,8.DOI:10.13702/j.1000-0607.2017.03.003
基于代谢组学研究热补针法治疗类风湿关节炎寒证家兔的作用机制
Mechanism of Heat-reinforcing Needling for Rheumatoid Arthritis Rabbits with Cold Syndrome Based on Metabonomics
摘要
Abstract
Objective To explore the specific mechanism of heat-reinforcing needling (HRN) for rheumatoid arthritis (RA) rabbits with cold syndrome based on metabolomics.Methods A total of 40 healthy rabbits were randomly divided into a normal group,a model group,a reinforcing-reducing needling(RRN) group,a twirling-reinforcing needling (TRN) group and a HRN group (n=8 rabbits/group).The RA model with cold syndrome was established with ovalbumin and freezing.Except the normal and model groups,RRN,TRN and HRN were given at "Zusanli" (ST 36) in the corresponding acupuncture groups for 30 min,once a day for 7 days.After the interventions,the pain threshold and the local skin temperature of each group were observed.The endogenous metabolites in the serum were analyzed by the gas chromatography mass spectrometry (GC-MS) technology combined with the pattern-recognition method.Results The pain threshold and the local skin temperature in the model group were lower than those in the normal group (P<0.05).The pain thresholds and the local skin temperatures in the three acupuncture groups were higher than those in the model group after interventions (P<0.05),and those in the HRN group were obviously higher than those in the RRN and TRN groups (P<0.05).The serum metabolites of leucine,valine,isoleucine,and arachidonic acid in the model group were increased compared with those in the normal group (P<0.05),and the metabolites of α-ketoglutaric acid,citric acid,succinic acid,glucose,inositol,palmitic acid,stearic acid,lactose,d-ribose,and D-mannose were decreased(P<0.05).The above-mentioned metabolites in the three acupuncture groups were significantly reversed relevant to the model group (P<0.05),and the effect of HRN group was significantly superior to those of the RRN and TRN groups in regulating α-ketoglutaric acid,citric acid,succinic acid,glucose,inositol,d-ribose ,and D-mannose (P<0.05).Conclusion HRN for RA with cold syndrome is effective,which may be related to the specific regulation for the krebs cycle and glycometabolism.关键词
热补针法/类风湿关节炎/代谢组学Key words
Heat-reinforcing needling/Rheumatoid arthritis/Metabonomics分类
医药卫生引用本文复制引用
杜小正,袁博,张星华,田亮,张婷卓,李兴兰,张枫帆,王金海..基于代谢组学研究热补针法治疗类风湿关节炎寒证家兔的作用机制[J].针刺研究,2017,42(3):202-208,216,8.基金项目
国家自然科学基金(No.81260558) （No.81260558）
国家中医药管理局甘肃郑氏针法学术流派传承工作室项目(No.2305135901) （No.2305135901）
