针刺研究2017，Vol.42Issue(3)：217-222,258,7.DOI:10.13702/j.1000-0607.2017.03.005
电针对局灶脑缺血/再灌注大鼠大脑缺血皮质区白介素-4/信号传导及转录激活因子6与核因子-κB的影响
Effect of Electroacupuncture Intervention on IL-4/STAT 6 and NF-κB p 65 Signaling in Cerebral Cortex in Focal Cerebral Ischemia/Reperfusion Injury Rats
摘要
Abstract
Objective To observe the effect of electroacupuncture (EA) on expression of cerebral cortical interleukin 4 (IL-4),signal transducer and activator of transcription 6 (STAT 6) and NF-κB family subunit p 65 in focal cerebral ischemia/reperfusion injury (CIRI) rats,so as to explore its mechanisms in reducing cerebral ischemia.Methods Forty-five male SD rats were randomly divided into sham group (n=15),CIRI model group (n=15),and EA group (n=15).The CIRI model was established by middle cerebral artery occlusion/reperfusion (MCAO/R).EA (1 mA,2 Hz/100 Hz) was applied to "Baihui"(GV 20) and the left "Hegu" (LI 4)-"Taichong" (LR 3) for 20 min,once every 12 h,three times altogether.The expression levels of p-STAT 6,STAT 6 and NF-κB p 65 in the ischemic cortex were detected by immunohistochemistry and Western blot,separately,and the content of IL-4 was detected by ELISA.Results Compared with the sham group,cerebral IL-4 content and the expression levels of NF-κB p 65 immunoactivity and protein were significantly up-regulated in the CIRI model group (P<0.001,P<0.01).Following EA intervention,the levels of IL-4,p-STAT 6 expression and ratio of p-STAT 6/STAT 6 were notably increased in the EA group than in the model group (P<0.01),while the levels of cerebral NF-κB p 65 immunoactivity and protein expression were significantly down-regulated (P<0.01,P<0.05).Conclusion EA of GV 20 and LI 4-LR 3 can up-regulate cerebral cortical IL-4 content and p-STAT 6 expression,and down-regulate NF-κB p 65 expression in CIRI rats,which may contribute to its effect in improving cerebral ischemia by reducing inflammatory injury.关键词
电针/局灶脑缺血/再灌注/大脑皮质/白介素-4/信号传导及转录激活因子/核因子-κB/炎性反应Key words
Electroacupuncture/Focal cerebral ischemia/reperfusion/Cerebral cortex/IL-4/STAT 6/NF-κB/Inflammatory injury分类
医药卫生引用本文复制引用
张莹,秦文熠,罗勇..电针对局灶脑缺血/再灌注大鼠大脑缺血皮质区白介素-4/信号传导及转录激活因子6与核因子-κB的影响[J].针刺研究,2017,42(3):217-222,258,7.基金项目
国家自然科学基金项目(No.30470606) （No.30470606）
国家自然科学基金青年基金(No.81403243) （No.81403243）
重庆市卫生局中医药科技项目(No.2012-2-128) （No.2012-2-128）
