针刺研究2017，Vol.42Issue(3)：235-239,5.DOI:10.13702/j.1000-0607.2017.03.008
麦粒灸对Lewis肺癌小鼠炎性微环境中白介素-6及信号转导蛋白和转录激活因子3的影响
Effect of Grain-moxibustion on IL-6 and STAT 3 in Inflammatory Microenvironment of Lewis Lung Cancer Mice
摘要
Abstract
Objective To observe the effect of grain-moxibustion on serum interleukin-6(IL-6) and signal transducers and activators of transcription 3 (STAT 3) in inflammatory microenvironment of Lewis lung cancer mice,so as to explore its possible mechanism underlying relieving Lewis lung cancer.Methods C 57 BL/6 mice were randomly divided into blank control group,model group,and moxibustion group (n=10 in each group).Lewis lung cancer model was established by hypodermic injection of Lewis tumor cell suspension.Mice in the moxibustion group were treated by grain-moxibustion at bilateral "Housanli"(ST 36),5 zhuang each acupoint,once a day for 10 days.The body weight,survival state score,volume of tumor,and survival rate were observed.Serum IL-6 level was detected by ELISA,and the expression of STAT 3 in tumor tissue was determined by Wes-tern blot.Results Compared with the blank control group,the body weight was significantly increased in the model group (P<0.05).The volume of tumor increased significantly in the model group,while it markedly decreased in the moxibustion group after 8 and 10 days treatment compared with the model group (P<0.05).The survival state of mice deteriorated companied with decreased survival rate in the model group,however,after 6,8 or 10 days treatment,the survival state scores were increased in the moxibustion group than in the model group (P<0.05).Compared with the blank control group,both serum IL-6 level and STAT 3 expression in tumor tissue were increased significantly in the model group (P<0.001).Compared with the model group,both serum IL-6 level and STAT 3 expression in tumor tissue were decreased significantly in the moxibustion group (P<0.001).Conclusion Grain-moxibustion has remarkable effect on Lewis lung cancer,by improving survival state,reducing death rate,and controlling growth rate of tumor,which might be associated with its function of inhibiting the activation of IL-6 and STAT 3 in inflammatory microenvironment.关键词
麦粒灸/Lewis肺癌/白介素-6/信号转导蛋白和转录激活因子3/炎性微环境Key words
Grain-moxibustion/Lewis lung cancer/IL-6/STAT 3/Inflammatory microenvironment分类
医药卫生引用本文复制引用
张雪,万茜,徐天舒..麦粒灸对Lewis肺癌小鼠炎性微环境中白介素-6及信号转导蛋白和转录激活因子3的影响[J].针刺研究,2017,42(3):235-239,5.基金项目
南京市医学科技发展项目(No.YKK12059、YKK11100) （No.YKK12059、YKK11100）
