针刺研究2017，Vol.42Issue(3)：240-245,6.DOI:10.13702/j.1000-0607.2017.03.009
点灸、电针对糖尿病胃轻瘫大鼠胃窦内皮细胞型一氧化氮合酶及血管紧张素Ⅱ mRNA表达的影响
Effect of Point-moxibustion and Electroacupuncture on the Expression of Endothelial Nitric Oxide Synthase mRNA and Angiotensin 2 mRNA in Gastric Antrum in Diabetic Gastroparesis Rats
摘要
Abstract
Objective To observe the effect of point-moxibustion and electroacupuncture(EA) on gastrointestinal motility,and expression of endothelial nitric oxide synthase(eNOS) mRNA and angiotensin 2(AT Ⅱ) mRNA in gastric antrum in diabetic gastroparesis(DGP) rats,so as to reveal the different effect between point-moxibustion and EA,and explore the mechanism underlying improvement of DGP.Methods Forty SD rats were randomly divided into normal,model,point-moxibustion and EA groups,10 rats in each group.The DGP model was established by intraperitoneal injection of streptozotocin (STZ,2%,55 mmol/kg) and fed with high-sugar and high-fat fodder."Zusanli" (ST 36),"Sanyinjiao" (SP 6) and "Liangmen" (ST 21) were chosen to be stimulated with moxibustion at 0,10 and 20 min in the point-moxibustion group or with EA for 20 min in the EA group.The treatment was conducted once daily for 15 days.After treatment,blood glucose,gastric emptying rate and small intestinal propulsive rate were measured.The plasma endothelin 1(ET-1) content was detected by ELISA.The mRNA expression of eNOS and AT Ⅱ in gastric antrum were detected by Real-time PCR.Results Compared with the normal group,the blood glucose in the model group was significantly higher (P<0.01).Compared with the model group,the blood glucose decreased significantly in the point-moxibustion group and EA group (P<0.05).In comparison with the normal group,gastric emptying rate and small intestinal propulsive rate were decreased,the content of plasma ET-1 was increased,the expression of eNOS mRNA in gastric antrum was significantly reduced and the expression of ATⅡ mRNA was significantly increased in the model group(P<0.01).In comparison with the model group,gastric emptying rate and small intestinal propulsive rate were increased,the content of plasma ET-1 was decreased,the expression of eNOS mRNA in gastric antrum was higher and the expression of AT Ⅱ mRNA was significantly lower in both point-moxibustion group and EA group (P<0.05),and there were no significant differences between the two treatment groups in above mentioned indexes (P>0.05).Conclusion Both point-moxibustion and EA can effectively promote gastrointestinal motility in DGP rats and improve the symptoms of delayed gastric emptying.The two therapies are effective,and their mechanism may be related to the increase of eNOS mRNA expression and the decrease of AT Ⅱ mRNA expression in gastric antrum.关键词
糖尿病胃轻瘫/点灸/电针/内皮细胞型一氧化氮合酶/血管紧张素ⅡKey words
Diabetic gastroparesis/Point-moxibustion/Electroacupuncture/Endothelial nitric oxide synthase/Angiotensin Ⅱ分类
医药卫生引用本文复制引用
刘丽,吴雪芬,郑雪娜,郭鑫,岳增辉,刘密,彭艳,袁建菱..点灸、电针对糖尿病胃轻瘫大鼠胃窦内皮细胞型一氧化氮合酶及血管紧张素Ⅱ mRNA表达的影响[J].针刺研究,2017,42(3):240-245,6.基金项目
国家重点基础研究发展计划(No.2014CB543102) （No.2014CB543102）
湖南省教育厅科学研究项目(No.14B129) （No.14B129）
国家自然科学基金项目(No.81673886、81403487) （No.81673886、81403487）
2016校创新性课题(No.2016CX26) （No.2016CX26）
