针刺研究2017，Vol.42Issue(3)：246-248,262,4.DOI:10.13702/j.1000-0607.2017.03.010
针刺对慢性疲劳综合征大鼠血浆T细胞转录因子/GATA结合蛋白3表达的影响
Effect of Acupuncture on the Expression of Transcription Factor T-bet/GATA-3 in Plasma of Rats with Chronic Fatigue Syndrome
摘要
Abstract
Objective To observe the effect of acupuncture on the expression of T-box expressed in T cell (T-bet)/ GATA binding factor-3 (GATA-3) in plasma of rats with chronic fatigue syndrome (CFS) and explore the mechanism of acupuncture treatment for CFS.Methods Forty-eight healthy male SD rats were randomly divided into blank control group,CFS model group,acupuncture group,and ginsenoside group (12 rats in each group).CFS rat model was established by combining restriction and cold water swimming.Acupuncture was applied to "Baihui"(GV 20), "Guanyuan" (CV 4) and "Zusanli" (ST 36, bilate-ral) acupoints,once a day for two weeks.The ginsenoside group was gavage administrated with ginsenoside,once a day for two weeks.After 14 days,behavioural changes were observed,and the expression levels of T-bet/GATA-3 genes in plasma were detected by RT-PCR.Results Compared with the blank control group,the time for immobility of forced suspensory test was signi-ficantly longer (P<0.05) and the time for exhaustive swimming was significantly shortened (P<0.05) in the CFS model group.Compared with the model group,the two indexes above-mentioned were reversed (P<0.05) both in the acupuncture group and the ginsenoside group,and the effects in the acupuncture group were more significant than those in the ginsenoside group (P<0.05).Compared with the blank control group,the expression level of T-cell transcription factor T-bet gene in plasma was higher in the CFS model group (P<0.05),companied with lower GATA-3 gene expression (P<0.05).The ratio of T-bet/GATA-3 was higher in the model group than in the blank control group(P<0.05).Compared with the CFS model group,all the indexes above-mentioned were reversed (P<0.05) in the two treatment groups.Acupuncture group showed a better effect on reducing T-bet gene expression than the ginsenoside group (P<0.05).Conclusion Acupuncture can decrease the expression level of T-bet gene while increase the expression of GATA-3 gene,which may be associated with its role in treating CFS.关键词
针刺/慢性疲劳综合征/转录因子T-bet/GATA-3Key words
Acupuncture/Chronic fatigue syndrome/Transcription factor T-bet/GATA-3分类
医药卫生引用本文复制引用
王向义,刘长征,雷波..针刺对慢性疲劳综合征大鼠血浆T细胞转录因子/GATA结合蛋白3表达的影响[J].针刺研究,2017,42(3):246-248,262,4.基金项目
江西省科技支撑计划资助项目(No.20142BBG70037) （No.20142BBG70037）
