中国康复理论与实践2017，Vol.23Issue(6)：628-633,6.DOI:10.3969/j.issn.1006-9771.2017.06.002
电针对完全性脊髓损伤后神经源性膀胱大鼠脊髓组织中Caspase-9、细胞色素C及凋亡蛋白酶激活因子-1表达的影响
Effect of Electroacupuncture on Expression of Caspase-9, Cytochrome C and Apoptotic Protease Activating Fac-tor 1 in Spinal Tissue in Rats with Neurogenic Bladder after Complete Spinal Cord Injury
摘要
Abstract
Objective To observe the effect of electroacupuncture on Caspase-9, cytochrome C (Cyt-C) and apoptotic protease activat-ing factor 1 (Apaf-1) protein in rats with neurogenic bladder after complete spinal cord injury. Methods A total of 60 female Sprague-Daw-ley rats were involved, in which 36 rats were randomly selected to establish the neurogenic bladder model with T10 spinal cord transected. 24 successful models selected were randomly divided into model group (n=12) and electroacupuncture group (n=12). The other 24 rats were al-so randomly divided into sham group (n=12) and blank group (n=12). The electroacupuncture group was electroacupunctured on Ciliao (BL32), Zhongji (RN3), Sanyinjiao (SP6) and Dazhui (GV14) for seven days. All of them were assessed with urodynamic test, TUNEL method was used to determine the apoptosis rate of the spinal cord, and Western blotting was used to detect the expression of Caspase-9, Cyt-C and Apaf-1 protein. Results Compared with the blank group and the sham group, the maximum bladder capacity and compliance de-creased (P<0.01), the urinary bladder pressure and leakage point pressure increased (P<0.05);the apoptosis rate of the spinal cord increased (P<0.001);the expression of Caspase-9, Cyt-C and Apaf-1 protein increased (P<0.05) in the model group and the electroacupuncture group. Compared with the model group, the maximum bladder capacity and compliance increased (P<0.01), the urinary bladder pressure and leak-age point pressure decreased (P<0.05); the apoptosis rate of the spinal cord decreased (P<0.05); the expression of Caspase-9, Cyt-C and Apaf-1 protein decreased (P<0.05) in the electroacupuncture group. Conclusion Electroacupuncture on Ciliao, Zhongji, Sanyinjiao and Da-zhui could improve the bladder function after spinal cord injury in rats. The mechanisms may be related with the down-regulation of Cas-pase-9, Cyt-C and Apaf-1 protein to reduce apoptosis.关键词
脊髓损伤/神经源性膀胱/电针/Caspase-9/细胞色素C/凋亡蛋白酶激活因子-1/凋亡/大鼠Key words
spinal cord injury/neurogenic bladder/electroacupuncture/Caspase-9/cytochrome C/apoptotic protease activating factor 1/apoptosis/rats分类
医药卫生引用本文复制引用
许明,张泓,刘继生,尹秀婷,张健,黄桂兰,艾坤..电针对完全性脊髓损伤后神经源性膀胱大鼠脊髓组织中Caspase-9、细胞色素C及凋亡蛋白酶激活因子-1表达的影响[J].中国康复理论与实践,2017,23(6):628-633,6.基金项目
国家自然科学基金面上项目(No.81473753). （No.81473753）
