中国康复理论与实践2017，Vol.23Issue(6)：634-640,7.DOI:10.3969/j.issn.1006-9771.2017.06.003
血清二胺氧化酶含量与大鼠急性脊髓损伤后肠道运动功能的关系
Relationship between Serum Diamine Oxidase Content and Intestinal Motility after Acute Spinal Cord Injury
摘要
Abstract
Objective To measure the level of diamine oxidase (DAO), and observe the intestinal motor and mucosal barrier injury after acute spinal cord injury (SCI) in rats. Methods A total of 45 Sprague-Dawley rats were randomly divided into SCI group (group A, n=15), sham group (group B, n=15) and control group (group C, n=15). SCI model was established with Allen's strike mode (10 g × 25 mm) by striking T10 spinal segment in rats. One day, three days and seven days after SCI, hind limb motor function was assessed with Basso-Beat-tie-Bresnahan (BBB) Scale in each group, the myoelectric slow wave and ileum smooth muscle contractility were measured in rats, ileum tis-sues were tested with HE staining, and the DAO content of serum was tested with ELISA kit. Results One day, three days and seven days af-ter SCI, the BBB score was significantly lower in group A than in groups B and C (P<0.001). One day, three days after SCI, the frequency and amplitude of both slow wave and contractility were lower in group A than in groups B and C (P<0.05);seven days after SCI, there was no significant difference among three groups (P>0.05). Group A showed ileal mucosal edema, lodging, inflammatory cell infiltration, and submucosal gap increase. The Chiu's score of intestinal mucosal injury was higher in group A than in groups B and C (P<0.05), as well as the serum DAO content one day and three days after SCI (P<0.05), and no significant difference was found in the serum DAO content among three groups seven days after SCI (P>0.05). Conclusion Serum DAO content may respond to the intestinal motor function and mu-cosal injury after acute SCI in rats.关键词
脊髓损伤/二胺氧化酶/肠道运动功能/黏膜损伤/大鼠Key words
spinal cord injury/diamine oxidase/intestinal motor function/mucosal injury/rats分类
医药卫生引用本文复制引用
杨森,杨拯,鄢靖欣,徐洲,徐文坚,田海燕,代小琳,杨平..血清二胺氧化酶含量与大鼠急性脊髓损伤后肠道运动功能的关系[J].中国康复理论与实践,2017,23(6):634-640,7.基金项目
1.四川省教育厅科研计划重点项目(No.16ZA0296) （No.16ZA0296）
2.成都医学院大学生创新实验计划项目(No.CXJS201309) （No.CXJS201309）
3.四川省科技厅应用基础研究项目(No.2015JY0205) （No.2015JY0205）
4.四川省卫生和计划生育委员会普及应用项目(No.17PJ591). （No.17PJ591）
