中国康复理论与实践2017，Vol.23Issue(6)：662-666,5.DOI:10.3969/j.issn.1006-9771.2017.06.009
有氧运动和膳食干预改善代谢综合征大鼠脂质代谢的效果及过氧化物酶体增殖物激活受体α介导的机制
Effect of Aerobic Exercises and Dietary Intervention on Lipid Metabolism in Rats with Metabolic Syndrome and Mechanism Medicated by Peroxisome Proliferator-Activated Receptorα
摘要
Abstract
Objective To study the effect of aerobic exercise and dietary intervention on lipid metabolism in metabolic syndrome rats, and investigate the possible mechanism mediated by peroxisome proliferator-activated receptorα(PPARα). Methods After one-week feed-ing, Sprague-Dawley rats were randomly divided into blank control group (CC group) and model group which were feed in high-fat-and-salt diet for 18 weeks to establish a metabolic syndrome model. Then, the metabolic syndrome rats were randomly divided into model control group (MC), the model high-fat diet group (MHE) and the model general died exercise group (ME). ME and MHE groups were forced to run on a treadmill for twelve weeks at the same time. The weight of perirenal fat, blood free fat acid (FFA), and blood lipid were determined. The expression of PPARαmRNA in myocardium was detected by RT-PCR. Western blotting was applied to detect the protein expression of PPARαin myocardium. Results Compared with CC group, MC group showed significant increase in body weight, perirenal fat weigh, FFA, and blood lipid (P<0.05), and significant decrease in PPARαmRNA and protein expression (P<0.01) in myocardium. Compared with MC group, ME and MHE groups showed significant decrease in body weight, perirenal fat weight, triglyceride (TG), and showed significant in-crease in high-density lipoprotein (HDL), and the expression of PPARαmRNA and protein in myocardium (P<0.05). Compared with MHE group, ME group showed decrease in low-density lipoprotein (LDL) (P<0.05), and increase in the expression of PPARαmRNA and protein (P<0.01). Conclusion Aerobic exercise may activate the expression of PPARα, enhance the utilization of fatty acid, reduce body mass and visceral fat mass, improve the dyslipidemia and then regulate lipid metabolism in metabolic syndrome rats.关键词
代谢综合征/过氧化物酶体增殖物激活受体α/有氧运动/膳食干预/脂代谢/大鼠Key words
metabolic syndrome/peroxisome proliferator-activated receptorα/aerobic exercise/dietary intervention/lipid metabolism/rats分类
医药卫生引用本文复制引用
张崇林,刘绍生,夏志,王世香,丁孝民,王前进,王卉..有氧运动和膳食干预改善代谢综合征大鼠脂质代谢的效果及过氧化物酶体增殖物激活受体α介导的机制[J].中国康复理论与实践,2017,23(6):662-666,5.基金项目
1.江西省教育厅科技计划项目(No.GJJ150772) （No.GJJ150772）
2.井冈山大学博士科研启动项目(自然科学) (No.JZB1314 （自然科学）
No.JZB11042). （）
