中国临床药理学与治疗学2017，Vol.22Issue(5)：490-494,5.
17-DMAG对鼻咽癌细胞株HNE1增殖与凋亡的影响
17-DMAG inhibits cell growth and induces apoptosis of nasopharynx cancer cell HNE1
摘要
Abstract
AIM:To explore the effect of 17-DMAG on nasopharynx cancer cell proliferation and apoptosis.METHODS:Cell viability of HNE1 was detected by MTT assay.Cell apoptosis of HNE1 was evaluated by flow cytometry with propidium iodide staining.JC-1 staining was used to determine the effect of 17-DMAG on mitochondrial membrane potential of HNE1 cells.The expressions of Bcl-2 and Bax were detected by Western blot.RESULTS:17-DMAG inhibited the cell proliferation of HNE1 cells in a dose and time-dependent manner.PI staining revealed that 17-DMAG significantly induced HNE1 cell apoptosis(P ＜ 0.01).JC-1 staining showed that 17-DMAG reduced the mitochondrial membrane potential of HNE1 cells.Western blot experiment showed that the expression of Bcl-2 decreased significantly (P ＜ 0.01).The expression of Bax increased significantly,especially in middle and high dose group after HNE1 cells being treated with 17-DMAG for 24 h (P ＜0.01).CONCLUSION:17-DMAG inhibits the growth of HNE1 cells in a dose and time-dependent manner by inducing the apoptosis of HNE1 cells.The mechanism is related with reducing the mitochondrial membrane potential and the expression of Bcl-2,and increases the level of Bax.关键词
17-DMAG/鼻咽癌/细胞增殖/凋亡Key words
17-DMAG/nasopharyngeal cancer/cell growth/apoptosis分类
医药卫生引用本文复制引用
王秀,刘健,张竞竞,朱娜,王清清,李见春..17-DMAG对鼻咽癌细胞株HNE1增殖与凋亡的影响[J].中国临床药理学与治疗学,2017,22(5):490-494,5.基金项目
国家自然科学基金资助项目(81372899) （81372899）
安徽省自然科学基金项目(1608085QH179) （1608085QH179）
安徽省高等学校自然科学研究项目(KJ20158065by) （KJ20158065by）
