中国临床药理学与治疗学2017，Vol.22Issue(5)：518-525,8.
链脲佐菌素诱导2型糖尿病大鼠合并脑缺血再注损伤模型优化方法的研究
Establishment of rat model of type 2 diabetes induced by streptozotocin combined with cerebral ischemic reperfusion injury
摘要
Abstract
AIM:To investigate an optimized rat model establishment of type 2 diabetes mellitus (T2DM) induced by streptozotocin (STZ) combined with cerebral ischemic reperfusion injury (T2DM-MCAO/R) so as to help the study of new drug for acute cerebral ischemia/reperfusion injury subjected to type 2 diabetes mellitus (T2DM).METHODS:High-fat and high-sugar (HFG) diet for 4 or 5 weeks were administered to induce insulin resistance,and STZ of different doses were injected to induce T2DM model.Models of T2DM-MCAO/R were built through five methods (A,B,C,D,E) with different doses of streptozotocin (STZ).Method A,B and C developed T2DM-MCAO/R injury models by surgery with sutures into brain from CCA;Method D and E,sutures from ECA.Method A administered drugs along with reperfusion;Method B and C injected drugs every day from reperfusion to 72 h;Method D and E,drugs were injected every day for two weeks before surgery.Syndrome,weight and fasting blood glucose (FBG) were assessed in various stages,and neurological behavioral scores were measured after the surgery to assess the quantity and quality of successful models.RESULTS:With method A-E,80％,77.8％,84.6％,100％,100％ rats were successfully modeled.While 93.3％ rats were successfully induced T2DM model by method E with the highest success rate and lowest death rate.Nimodipine and insulin could all improve the fasting blood glucose level,neurological deficits and reduced infarction volume percentage at the same time.CONCLUSION:Rat model of T2DM-MCAO/R injury established by two times injection of low dose STZ and MCAO/R surgery (introduced from ECA) present higher successful modeling rate,lower death rate and better stability.It is referential to investigate the effect and mechanism of new medicine for preventing and treating ischemia/reperfusion injury subjected to T2DM.关键词
2型糖尿病/链脲佐菌素/再灌注损伤Key words
type 2 diabetes mellitus/streptozotocin/reperfusion injury分类
医药卫生引用本文复制引用
刘畅,姜道利,刘小芳,徐佳,王瓅珏,王茜,许玲,方翼..链脲佐菌素诱导2型糖尿病大鼠合并脑缺血再注损伤模型优化方法的研究[J].中国临床药理学与治疗学,2017,22(5):518-525,8.基金项目
北京大学人民医院研究与发展基金(RDB2013-22) （RDB2013-22）
