中国临床药理学与治疗学2017，Vol.22Issue(5)：526-530,5.
姜黄素靶向抑制SW480细胞信号通路研究
Effect of curcumin on the human colon cancer cells SW480
摘要
Abstract
AIM:To study the effect of curcumin on human colon cancer cells SW480.METHODS:Cell viability and the effects of curcumin on the activity of SW480 cells were detected by MTS method.Expressions of β-catenin and TCF4 were detected by Western blot.E-cadherin and Vimentin by real-time qPCR assay in human colon cancer cells SW480.RESULTS:Curcumin could significantly inhibit the proliferation of SW480 cells and the IC50 were (75.75 ±3.17) μmol/L(12 h),(21.15± 2.47) μmol/L(24 h),(18.95 ± 1.75) μmol/L (48 h),(17.93 ±1.55) μmol/L(72 h).Curcumin could up-regulate the expression of E-cadherin,and down-regulate the expression of Vimentin,β-catenin and TCF4 in SW480 cells.CONCLUSION:Curcumin can inhibit the the activity of SW480 cells through the Wnt/β-catenin signaling pathway.关键词
姜黄素/SW480细胞/β-catenin/T细胞转录因子4/抑制Key words
curcumin/SW480 cells/β-catenin/TCF4/inhibition分类
医药卫生引用本文复制引用
陈永财,周斌,陈雪敏,翁金月..姜黄素靶向抑制SW480细胞信号通路研究[J].中国临床药理学与治疗学,2017,22(5):526-530,5.基金项目
浙江省中医药资助项目(2015AZ195) （2015AZ195）
