中国临床药理学杂志2017，Vol.33Issue(9)：794-797,4.DOI:10.13699/j.cnki.1001-6821.2017.09.007
丹参多酚酸对心肌缺血大鼠血清及心脏组织炎症因子的保护作用
Protective effects of Salvianolic acid on myocardial ischemic injury of rats from the aspect of inhibiting inflammatory reaction
摘要
Abstract
Objective To investigate the protective effects of Salvianolic acid on myocardial ischemic injury of rats from the aspect of inhibiting inflammatory reaction.Methods Sixty Sprague Dawley (SD) male rats were randomly divided into four groups (n =15per group):sham operation group,model group,experimental-high (36 mg · kg-1) group,experimental-low (18mg · kg-1) group with intraperitoneal injection dosing.Same amount of distilled water was injected to sham operation group and model group,lasting for 4 days.Myocardial ischemia model was established by coronary artery ligation of left anterior descending (LAD) branch after the last administration.Six hours after the operation,the blood of eye canthus was collected to assay the levels of creatine kinase(CK),MB isoenzyme of creatine kinase (CK-MB) and lactate dehydrogenase (LDH) in serum.The heart was collected 24 h after the operation,stained by hematoxylin eosin (HE)and checked the pathological change of the cardiac muscle tissue,assayed the cardiac troponin (cTnT),tumor necrosis factor α (TNF-α) in serum and myeloperoxidase (MPO) was measured in myocardial tissue homogenate.Results After 6 hours' myocardial ischemia,the serum CK in sham operation group was (529.61 ± 141.93) U · L-1,CK-MB was (708.12 ±385.93) U · L-1,LDH was (330.12 ±158.38) U · L-1 and cTnT was (294.12 ±55.10)pg· mL-1 after 24 hours' myocardial ischemia.The serum CK in model group was (996.42 ± 413.42) U · L-1,CK-MB was (1346.11 ±558.30) U · L-1,LDH was (520.12 ±154.76) U · L-1and cTnT was (513.00 ±69.71)pg · mL-1after 24 hours' myocardial ischemia.The serum CK in experimental-low and experimental-high groups were (499.45 ±159.33),(514.91 ±98.82)U · L-1,CK-MB were (831.42 ±385.11),(592.10 ±206.32)U·L-1,LDH were (462.62 ±229.68),(437.72 ± 175.80)U· L-1,and cTnT were (431.12 ±106.00),(338.80 ± 76.92)pg · mL-1after 24 hours' myocardial ischemia.Compared with the sham operation group,the CK,CK-MB and LDH value in the serum of model group increased significantly (P ＜ 0.05).Compared with the model group,the salvianolic acid significantly both can decrease the CK and CK-MB in serum (P ＜ 0.05).Compared with sham operation group,the cTnT value in the serum of model group increased significantly (P ＜ 0.05).Compared with model group,high dosage of salvianolic acid could significantly decrease the cTnT value (P ＜ 0.05).After 24 hours'myocardial ischemia,the TNF-α in serum of sham operation group was (118.90 ± 17.58) pg · mL-1,the MPO in heart tissue was (32.25 ± 3.75) U · L-1.The TNF-α in serum of model group was (156.00 ± 28.24) pg · mL-1,the MPO in heart tissue was (104.83 ±22.87) U · L-1.The TNF-α in serum of experimental-low and experimental-high groups were (136.05 ± 34.00),(125.06 ± 30.13) pg · mL-1,the MPO in heart tissue were (91.70 ±21.57),(68.00 ± 18.47) U · L-1.Compared with sham operation group,the TNF-α values in the serum and the MPO value in model group increased significantly (P ＜ 0.05).Compared with model group,these values of experimental-high group decreased with significantly (all P ＜ 0.05).Conclusion Salvianolic acid showed the protection of myocardial tissue,which also could significantly reduce the myocardial cell injury and inflammatory cell infiltration of cardiac myocytes,which could be related with the decreased levels of TNF-α and MPO.关键词
丹参多酚酸/心肌缺血/炎性因子/心肌保护Key words
Salvianolic acid/myocardial ischemia/inflammatory cytokine/myocardial preservation分类
医药卫生引用本文复制引用
秦袖平,许梦习,郝荣荣,张密霞,庄朋伟,崔广智,张艳军..丹参多酚酸对心肌缺血大鼠血清及心脏组织炎症因子的保护作用[J].中国临床药理学杂志,2017,33(9):794-797,4.基金项目
科学技术部重大新药刨制专项基金资助项目(2012zx09101202) （2012zx09101202）
科学技术部长江学者和创新团队发展计划基金资助项目(IRT_14R41) （IRT_14R41）
