中国临床药理学杂志2017，Vol.33Issue(9)：802-805,4.DOI:10.13699/j.cnki.1001-6821.2017.09.009
炎症状态下阿托伐他汀对HepG2细胞氧化应激和脂质积聚的影响
Effect of atorvastatin on oxidative stress and intracellular lipid accumulation under inflammatory stress in HepG2 cells
摘要
Abstract
Objective To investigate the effect of atorvastatin on oxidative stress and intracellular lipid accumulation in HepG2 cells under inflammatory state and explore the underlying mechanism.Methods HepG2 cells were treated with 100 ng · mL-1 TNF-α,100 ng · mL-1 TNF-α ± 10 μmol · L-1 atorvastatin in the presence of LDL for 24 h.Oil red O staining was used to examine the intracellular lipid contents.The mRNA and protein expressions of lipogenic genes (FAS,ACC and SREBP1) were detected by real-time polymerase chain reaction and Western blot.ROS levels were measured with the fluorescent probe of DCFH-DA.Contents of H2O2 and MDA were determined using the colorimetric method.Results Compared with normal group(the gray value of SREBP1 was 1.01 ± 0.001),the gray value of SREBP1 in model group was 1.61 ± 0.34.The mRNA levels in normal group of SREBP1,FAS,ACC respectively were 1.01 ± 0.16,1.03 ± 0.32,0.95 ± 0.29,the values in model group respectively were 3.61 ± 0.39,1.99 ± 0.36,2.37 ± 0.52,the differences were statistically significantly (P ＜ 0.05).Compared with model group,the mRNA levels of SREBP1,FAS,ACC and the gray value of SREBP1 in experimental group respectively were 2.95 ± 0.92,3.99 ± 1.16,2.85 ± 0.91,2.94 ± 0.65,the differences were statistically significantly(P ＜0.05).At the same time,compared with normal group,the levels of ROS(fluorescenceintensity),H2O2,MDA respectively were 1.00 ±0.20,and (2.30 ±0.31) (0.78 ±0.22) nmol · mg-1,the levels in model group respectively were 1.77 ± 0.25 and (4.32 ± 0.77),(1.86 ± 0.23) nmol · mg-1,the differences were statistically significantly (P ＜ 0.05).Compared with model group,the levels of ROS,H2 O2,MDA in HepG2 cells in experimental group respectively were 3.2 ±0.53 and (5.31 ±0.75),(3.43 ± 1.15) nmol · mg-1,the differences were statistically significantly(P ＜ 0.05).Conclusion Atorvastatin induced intracellular lipid accumulation in HepG2 cells under inflammatory stress,which may be associated with the increased oxidative stress.关键词
阿托伐他汀/炎症应激/脂质积聚/人肝癌细胞株Key words
atorvastatin/inflammatory stress/lipid accumulation/HepG2 cell分类
医药卫生引用本文复制引用
肖亚运,吴为,周晓倩,陈压西,阮雄中,赵蕾..炎症状态下阿托伐他汀对HepG2细胞氧化应激和脂质积聚的影响[J].中国临床药理学杂志,2017,33(9):802-805,4.基金项目
国家自然科学基金资助项目(31540029) （31540029）
重庆市第七批市科技计划基金资助项目(cstc2016jcyjA0545) （cstc2016jcyjA0545）
