中国临床药理学杂志2017，Vol.33Issue(9)：810-812,3.DOI:10.13699/j.cnki.1001-6821.2017.09.011
冈田酸抑制胰腺癌细胞株PANC-1迁移及其增殖作用
Inhibition effect of kadaic acid on migration and proliferation in pancreatic cancer cell line PANC-1
摘要
Abstract
Objective To study the function of protein phosphatase 2A (PP2A) inhibitor (kadaic acid,KA) on migration and proliferation in pancreatic cancer cell line PANC-1.Methods PANC-1 cells were divided into four groups:control group,experimental-Ⅰ group,experimental-Ⅱ group,experimental-Ⅲ group.The experimental-Ⅰ group Ⅰ was treated with KA,experimental-Ⅱ group was treated with Wnt/β-catenin pathway inhibitor(FH535).In experimental-Ⅲ group was treated with KA + FH535.The cell mobility was assessed by photomicrography.And cell viability was assessed using the panel absorbance ratio.Results The mobility of group Ⅰ and control group were 16.28％,18.13％;21.94％,25.34％;27.67％,70.64％,respectively at the 2,4,6 h,compared with control group,the difference had significance(P ＜ 0.05).Which indicated that KA could inhibit the migration of pancreatic cancer cell line PANC-1.In experimental-Ⅱ group,the migration rate was significantly lower than that in control group (P ＜ 0.05).The cell activity in experimental-Ⅱ group and experimental-Ⅲ group were 52.39％,51.81％;49.57％,30.67％;30.64％,12.57％;15.91％,3.59％,respectively at 0,6,12,18 h.The migration of β-catenin at the time points of 0,6,12,18 h was significantly lower than that of the control group,and the difference was statistically significant (P ＜ 0.05).Which indicated that KA could down-regulate the activity of Wnt/β-catenin pathway in pancreatic cancer cells.The inhibitory effect of KA on migration and proliferation of pancreatic cancer cell line was inhibited by FH535.And the inhibitory effect of KA on the migration and proliferation of pancreatic cancer cell line PANC-1 may be based on the Wnt / β-catenin signal pathway.Conclusion KA can inhibit the migration and proliferation of pancreatic cancer cell line PANC-1.The mechanism may be that the inhibition of Wnt/ β-catenin signal pathway is down-regulated.关键词
PP2A抑制剂/冈田酸/胰腺癌细胞株/迁移及增殖Key words
protein phosphatase 2A inhibitor/kadaic acid/pancreatic cancer cell line/migration and proliferation分类
医药卫生引用本文复制引用
高瑞灏,秦晓青,李贵新..冈田酸抑制胰腺癌细胞株PANC-1迁移及其增殖作用[J].中国临床药理学杂志,2017,33(9):810-812,3.基金项目
山东省自然科学基金资助项目(2014ZRB14028) （2014ZRB14028）
