中国临床药理学杂志2017，Vol.33Issue(9)：813-816,4.DOI:10.13699/j.cnki.1001-6821.2017.09.012
塞来昔布对颅脑创伤后大鼠的凋亡蛋白Bcl-2和Bax表达影响
Effects of celecoxib on expression of apoptosis protein Bcl-2 and Bax after craniocerebral injury in rats
摘要
Abstract
Objective To investigate the effects of celecoxib (selective cyclooxygenase-2 inhibitor) on the expression of Bcl-2 and Bax in rat brain after severe craniocerebral injury in rats.Methods SD rats were randomly divided into four groups:experimental group,model group,sham operation group and control group.The rats in experimental group and model group were established with closed craniocerebral injury model.In the experimental group,intraperitoneal injection of celecoxib (250 mg · kg-1) was performed immediately after the craniocerebral injury model was established.The rats in sham operation group and the control group were given the same amount of 0.9％ NaCl intraperitoneally.The expressions of Bcl-2 and Bax mRNA and protein were detected by immunohistochemical staining.Results The expression of Bcl-2 protein in the experimental group,model group,sham-operated group and control group were 68.75 ± 0.62,45.40 ± 0.36,81.08 ± 0.65,78.12 ±0.05.The expression of Bcl-2 in the model group was significantly lower than in sham-operated group and control group with significantly (P ＜ 0.05).Compared with the model group,the expression of Bcl-2 in the experimental group decreased with significantly (P ＜ 0.05).The expression of Bax protein in experimental group,model group,sham operation group and control group respectively were 68.28 ±0.35,92.48 ±0.16,65.06 ±0.63,63.15 ±0.10.The expression of Bax in model group was significandy higher than in sham-operated group and control group with significantly (P ＜ 0.05).Compared with the model group,the expression of Bax in the experimental group decreased with significantly(P ＜ 0.05).Conclusion Celecoxib can increasing the expression of Bcl-2,decreasing the expression of Bax,inhibiting the apoptosis of neurons after raniocerebral injury,has a protective effect on the traumatic brain injury in rats.关键词
塞来昔布/颅脑损伤/B淋巴细胞瘤-2/B淋巴细胞瘤-2-相关X蛋白Key words
celecoxib/craniocerebral trauma/B-cell lymphoma-2/Bcl-2-associated X protein分类
医药卫生引用本文复制引用
张涛,国建飞,邢琳琳,张金玲,李喜朋,张宇新..塞来昔布对颅脑创伤后大鼠的凋亡蛋白Bcl-2和Bax表达影响[J].中国临床药理学杂志,2017,33(9):813-816,4.基金项目
河北省自然科学基金资助项目(C2004000689) （C2004000689）
河北省博士基金资助项目(05547008D-4) （05547008D-4）
河北省科学技术与社会发展计划基金资助项目(04276135) （04276135）
