中国临床药理学杂志2017，Vol.33Issue(9)：821-824,4.DOI:10.13699/j.cnki.1001-6821.2017.09.014
离子交换色谱法测定健康人血浆和尿中的盐酸二甲双胍浓度
Determination of metformin hydrochloride tablets in healthy plasma and urine by ion exchange chromatography
杨欢杰 1张海波 2徐蕊 1高惠静 1袁圆 2赵军1
作者信息
- 1. 新疆医科大学第一附属医院药学部,乌鲁木齐830011
- 2. 新疆医科大学药学院,乌鲁木齐830011
- 折叠
摘要
Abstract
Objective To develop an ion exchange chromatography method for determination of metformin in human plasma,and further apply the method in pharmacokineties study.Methods Human plasma was precipitated with acetonitrile containing internal standard (chlorpheniramine maleate),after mixing and centrifugation,the supernatant requried nitrogen drying at 40 C of water bath,and then the residue was redissolved by mobile phase,after mixing and centrifugation,then injected the supernatant to analyzed on column.Using Capcell PAK SCX UG80 (4.6 mm × 150 mm,5 μm) column.The mobile phase,consisting of acetonitrile and 0.15 mol · L-1 ammonium dihydrogen phosphate (adjusted to pH 3 with 10％ phosphoric acid solution) (52∶ 48,v/v).The samples were eluted at a flow rate of 1 mL · min-1,35 ℃ of column temperature and assessed by a UV detector set at 233 nm.Urine samples were diluted by ultra pure water,then analyzed by HPLC.Results The calibration curve in plasma and urine samples exhibited a linear dynamic range of 0.05-5 μg · mL-1 (r =0.999 3) and 0.20-200 μg · mL-1 (r =0.999 1) respectively.The limit of quantification were 0.05,0.20μg · mL-1 respectively.The extraction recoveries were 88.65％-95.42％in serum and 91.22％-108.18％ in urine.The inter-day and intra-day RSDs were less than 15％.And the plasma and urine samples were all in good stability.Conclusion This method is widely applicable,strong specificity,good reproducibility and high sensitivity,it is suitable for the pharmacokinetic study of metformin hydrochloride tablets.关键词
盐酸二甲双胍/离子交换色谱法/血药浓度/药代动力学Key words
metformin hydrochloride/ion exchange chromatography/human plasma/pharmacokinetics分类
医药卫生引用本文复制引用
杨欢杰,张海波,徐蕊,高惠静,袁圆,赵军..离子交换色谱法测定健康人血浆和尿中的盐酸二甲双胍浓度[J].中国临床药理学杂志,2017,33(9):821-824,4.
