中国临床药理学杂志2017，Vol.33Issue(9)：825-827,831,4.DOI:10.13699/j.cnki.1001-6821.2017.09.015
液质联用法检测人血浆中舒芬太尼浓度
HPLC-MS/MS method for the determination of sufentanil in human plasma
刘维 1熊歆 2张现化 1赵艳 2吴琼 1翟所迪2
作者信息
- 1. 北京大学第三医院药剂科,北京100191
- 2. 北京大学治疗药物监测与临床毒理中心,北京100191
- 折叠
摘要
Abstract
Objective To establish a sensitive,rapid and robust high performance liquid chromatography-mass spectrometry (HPLC-MS/MS) method for the analysis of sufentanil in human plasma.Methods Sufentanil in human plasma was extracted by tert-butyl methyl ether and analyzed by HPLC-MS/MS with fentanyl as the internal standard (IS).Multiple reactions monitoring was used,transitions and collision energies were as follows:sufentanil m/z 387.2→m/z 238.2(27 V),fentanyl m/z 337.2→m/z 158.2 (37 V) in positive electrospray ionization mode.The chromatographic separation was performed on Waters Atlantis Hilic Silica column (2.1 mm ×-50 mm,5 μm),with a flow rate at 0.3 mL · min-1.A gradient elution was applied,mobile phase was 10 mmol · L-1 ammonium formate grid acetonitrile,both containing 0.1％ formic acid.Results The calibration curve was y =9.28 × 10-4x + 1.86 × 10-3 (r =0.997 4),over the concentration range of 2-500 pg · mL-1 was linear,and the lower limit of qualification (LLOQ) was 2 pg · mL-1.The intra-day and inter-day precisions were all less than 10％.Conclusion The assay was successfully applied to the analysis of sufentanil in patients which can provide reliable results.关键词
舒芬太尼/芬太尼/液质联用/血浆Key words
sufentanil/fentanyl/HPLC-MS/MS/plasma分类
医药卫生引用本文复制引用
刘维,熊歆,张现化,赵艳,吴琼,翟所迪..液质联用法检测人血浆中舒芬太尼浓度[J].中国临床药理学杂志,2017,33(9):825-827,831,4.
