中国临床药理学杂志2017，Vol.33Issue(9)：828-831,4.DOI:10.13699/j.cnki.1001-6821.2017.09.016
高效液相色谱法同时测定人全血中阿糖胞苷及其代谢物阿糖尿苷的浓度
Determining the concentration of cytosine arabinoside and its metabolites 1-β-D-arabinofuranosyluracil in human blood by HPLC
摘要
Abstract
Objective To establish a method for simultaneous determining the concentration of cytosine arabinoside (Ara-C) and 1-β-D-arabinofuranosyluracil (Ara-U) in human blood by HPLC-UV.Methods Both Ara-C and Ara-U were extracted from blood by protein deposition method and determined by HPLC,and separated on a Cosmosil Cholester(4.6 mm ×250 mm,5 μm) with mobile phase consisted of methanol∶ (0.01 mol · L-1,pH =6.7) phosphate buffer solution (3∶ 97) at a flow rate of 1 mL · min-1.The detective wavelength was 280 nm and the column temperature was maintained at 25 ℃.Results]he linear range of Ara-C was 1.55-198.00 μg · mL-1 (r =0.9985) and Ara-U was 1.34-171.00 μg · mL-1 (r =0.997 8) with the formulation y =16417.59x-14511.01 and y =4468.72x-1521.15 respectively.When S/N≥3,the detection limit of Ara-C and Ara-U were 0.79,0.67 μg · mL-1.The recovery rate of Ara-C and Ara-U was respectively 74.84％-87.74％ and 77.76％-88.92％,with both intra-day RSD less than 15％ and about inter-day,the high and medium concentration RSD both were less than 15％ and 20％.Conclusion The established method was accurate and specific,meanwhile,it has high recovery,litde interference and low cost,thus it's applicable for the clinical determination of blood concentration of Ara-C and Ara-U.关键词
高效液相色谱法/阿糖胞苷/阿糖尿苷/血药浓度Key words
high performance liquid chromatography/cytosinearabinoside/1-β-D-arabinofuranosyluracil/blood drug concentration分类
医药卫生引用本文复制引用
高娟,王捷,张奇洲,买雪婷,滕亮..高效液相色谱法同时测定人全血中阿糖胞苷及其代谢物阿糖尿苷的浓度[J].中国临床药理学杂志,2017,33(9):828-831,4.基金项目
新疆医科大学研究生创新基金资助项目(CXCY061) （CXCY061）
