中国临床药理学杂志2017，Vol.33Issue(10)：893-896,4.DOI:10.13699/j.cnki.1001-6821.2017.10.008
注射用丹参多酚酸对胶质细胞神经营养因子及神经元的保护作用
Protective effect of Salvianolate injection on astrocytic neurotrophic factors and the Neuro-2A
摘要
Abstract
Objective To investigate the role of Salvianolate injection on the nerve growth factor transcription synthesis and secretion effect in glial cells C8-D1A induced by oxygen glucose-deprivation (OGD),in addition,the protective effect of astrocytic cell (C8-D1A) conditioned medium treated by Salvianolate on oxygen glucose-deprived Neuro-2A were also explored.Methods The glial cells were divided into control group,model group,Salvianolate group,Salvianolic acid B group and Salvianolic acid D group.The control group was changed into high glucose buffer solution,and cultured for 4 h.The model group cells were sealed in a hypoxic chamber,and the cells were cultured in the incubator at 37 ℃ for 4 h.After 4 h,the whole serum-free medium was added.The cells in model group were exposed to hypoxia and hypoglycemia for 4 h,then changed to different concentrations (0.1,1,10,50 μg · mL-1) of Salvianolate injection and Salvianolic acid B and Salvianolic acid D in serum-free medium.After 24 h,the viability of the cells was detected by CCK-8,the brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) in glial cells were detected by the enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR).Neuro-2A cells were treated as well as C8-D1A cells in the control,model and drug groups.After 4 h,the supernatant of the Neuro-2A control group was replaced by the glial cell supernatant,the supernatant of Neuro-2A model group was replaced by the supernatant of the glial cell model group,the supernatant of Neuro-2A drug group was replaced by the supernatant of the glial cell drug group.The viability of Neuro-2A cells was also detected by CCK-8.Results Compared with control group (1.00 ± 0.08),Salvianolate injection groups (0.1,1,10,50 μg· mL-1) were 1.04 ±0.09,1.05 ±0.09,1.03 ±0.07,1.02±0.06,had no cytotoxicity on C8-D1A cell viability,and Salvianolic acid B could significantly promoted C8-D1A cell proliferation(1.05 ±-0.14).Salvianolate injection had promoted synthesis and secretion of nerve growth factor in C8-D1A cell,but had no statistical difference (P ＞ 0.05).On Neuro-2A cell viability study,compared with control group(0.57 ± 0.05),Neuro-2A model group cell viability(0.49 ± 0.40) were decreased significantly treated by the conditional medium from glial model group.Furthermore,compared with model group (0.49 ± 0.40),the 10 μg · mL-1 Salvianolate injection group (0.85 ± 0.02) can significantly improve the vitality of Neuro-2A (P ＜ 0.05).Conclusion Salvianolate treated glial conditioned medium have a protective effect on hypoxia Neuro-2A cells.关键词
注射用丹参多酚酸/脑源性神经营养因子/胶质细胞源性神经营养因子Key words
Salvianolate injection/brain-derived neurotrophic factor/glial cell-derived neurotrophic factor分类
医药卫生引用本文复制引用
袁庆,胡利民,王少峡,张玥,郭虹,赵莼,徐杨杨,柴丽娟..注射用丹参多酚酸对胶质细胞神经营养因子及神经元的保护作用[J].中国临床药理学杂志,2017,33(10):893-896,4.基金项目
国家科技重大专项基金资助项目(2012zx09101202) （2012zx09101202）
国家自然科学基金资助项目(81573644) （81573644）
国家“重大新药创制”科技重大专项基金资助项目(2011ZX09201) （2011ZX09201）
