中国临床药理学杂志2017，Vol.33Issue(10)：901-904,4.DOI:10.13699/j.cnki.1001-6821.2017.10.010
熊果酸对胰腺癌细胞SW1990增殖及其对微小RNA-760表达的影响
Effect of ursolic acid on the proliferation of pancreatic cancer cells SW1990 and the expression of its small RNA-760
摘要
Abstract
Objective To investigate effect of ursolic acid on the proliferation of pancreatic cancer cells SW1990 and the expression of its small RNA-760 (miR-760).Methods The SW1990 cells were divided into control group and test Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅴ groups.Control group was given a blank medium solution;test Ⅰ,Ⅱ,llⅢ,Ⅳ,Ⅴ groups were givencultured medium solution containing ursolic acid 5,10,20,40,80 μmol · L-1,respectively.After 24,48,72 h,the proliferation of cells were measured by tetrazolium salt (MTT);changes in cell morphology were observed by inverted microscope;the expression levels of miR-760 was determined by extraction kit and reverse transcription polymerase chain reaction.Results The cell survival rates of control group and test Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅴ groups after 24 h were (100.21 ± 2.89) ％,(94.42 ± 2.24) ％,(90.52 ± 2.13) ％,(86.71 ± 0.48) ％,(66.11 ± 1.46) ％,(40.50 ± 1.32) ％,respectively;after 48 h,cell survival rates were (100.38 ± 2.56)％,(91.09 ± 2.36)％,(85.54 ± 7.43)％,(73.56 ± 9.01)％,(52.72 ± 1.25)％,(33.85 ± 1.27) ％,respectively;after 72 h,cell survival rates were (101.52 ± 2.42) ％,(84.66 ± 2.51) ％,(73.75 ±6.27)％,(63.10±2.08)％,(44.77 ±1.77)％,(24.43 ±1.30)％ respectively;after 24 h,the expression of miR-760 were 1.00 ±0,1.15 ±0.23,1.47 ±0.41,1.77 ±0.27,2.65±0.14,3.54 ±0.51,respectively;after 48 h,the of expression miR-760 were 1.00 ±0,1.27 ±0.15,1.75 ±0.23,2.24 ±0.31,3.52 ±0.22,4.43 ± 0.31,respectively;after 72 h,the expression of miR-760 were1.00 ± 0,1.40 ± 0.11,2.00 ± 0.10,2.52 ± 0.17,3.93 ± 0.65,5.16 ± 0.45,respectively,ursolic acid groups had statistically significant differences compared with the control group (P ＜ 0.05).With the increase of the concentration of ursolic acid and the prolongation of action time,SW1990 cells gradually exhibit deformation,shrink,or even death,miR-760 levels increased.Conclusion Ursolic acid has a significant value-added inhibition on the pancreatic cancer cells in a dose and time dependent,and a high concentration of ursolic acid under a prolonged action time may result in an increased expression of miR-760 cells.关键词
熊果酸/胰腺癌/SW1990细胞/细胞增殖/微小RNA-760Key words
ursolic acid/pancreatic cancer/SW1990 cell/proliferation/small RNA-760分类
医药卫生引用本文复制引用
马红梅..熊果酸对胰腺癌细胞SW1990增殖及其对微小RNA-760表达的影响[J].中国临床药理学杂志,2017,33(10):901-904,4.基金项目
浙江省中医药科学研究基金资助项目(2015ZA101) （2015ZA101）
