中国临床药理学杂志2017，Vol.33Issue(10)：917-920,938,5.DOI:10.13699/j.cnki.1001-6821.2017.10.014
煎煮形式及配伍比例对党参黄芪复方免疫调节作用的影响
Effect of decoction forms and compatibility proportion of Codonopsis pilosula and Astragalus membranaceus compound on immunomodulatory
摘要
Abstract
Objective To study the effect of decoction forms and compatibility proportion of Codonopsis and Astragalus compound on immunomodulatory.Methods The kunming mice were randomly divided into nine groups:normal,model,control,test-Codonopsis,test-Astragalus,test-merged with individual decocted,test-Codonopsis and Astaagalus (1 ∶ 1),(1 ∶ 2),(2 ∶ 1),each group had ten rats.Making low-immunity model mice by cyclophosphamide (CTX 40 mg · kg-1).The normal and model were administrated the same quantity of normal saline,the control were administrated the Shenqi particle (5 g · kg-1),test-Codonopsis,test -Astragalus,test-merged with individual decocted,test-Codonopsis and Astragalus (1 ∶ 1,1 ∶ 2,2∶1) were administrated the corresponding equal doses test agent(14.3 g · kg-1)for 15 days.The visceral index was counted by weighted.The peritoneal macrophage phagocytic count by intraperitoneal injection 5 ％ chicken red blood-cell(CRBC) were meaeured(OD value).The serum hemolysin OD through delayed type hypersensitivity maded by 50％ dinitrochlorobenzene (DNCB).The reticuloendothelial system phagocytic count were meaeured by caudal vein injection carbon inkl.Results Compared with model group (thymus index 0.64 ± 0.24 g · kg-1,spleen index 1.83 ± 0.89 g · kg-1),the thymus index (g · kg-1) in test-Codonopsis,test-Astragalus,test-merged with individual decocted,test-Codonopsis and Astragalus (1 ∶ 1,1 ∶ 2,2∶1) were 1.23 ± 0.73,1.42 ± 0.43,1.47 ± 0.41,1.97 ± 1.05,1.82 ± 0.99,1.49 ± 0.59,all can varying degrees improve with siginificantly (P ＜ 0.05 or P ＜ 0.01) and spleen index (g · kg-1) in above groups were 2.09 ± 2.61,2.38 ± 1.43,2.40 ± 1.39,2.43 ± 1.44,2.58 ± 1.05,2.36 ± 0.89 with siginificantly (P ＜0.05 or P ＜0.01).Compared with model group,the peritoneal macrophage phagocytic count(％)in above groups were 30.41 ±7.28,39.96 ± 10.45,42.23 ±7.43,44.83 ±7.95,43.75 ± 10.60,40.43 ± 8.91 and the reticuloendothelial system phagocytic countin above groups were 0.04 ± 0.03,0.04 ± 0.04,0.06 ± 0.02,0.07 ± 0.04,0.06 ±0.03,0.04 ± 0.02 with siginificantly (P ＜ 0.05 or P ＜ 0.01).Compared with model group (OD value 0.24 ± 0.05,0.28 ± 0.11),the restrain delayed type hypersensitivityin above groups were 0.37 ± 0.16,0.40 ± 0.12,0.42 ±0.17,0.50 ±0.25,0.46 ±0.17,0.44 ±0.20 in immunosuppressed mice induced by CTX and serum hemolysin OD in above groups were 0.58 ±0.08,0.64 ±0.11,0.48 ±0.14,0.68 ±0.08,0.54 ±0.18,0.64 ±0.06 with siginificantly (P ＜0.05 or P ＜0.01).Comparatively speaking,the effect of test-Codonopsis and Astragalus (1∶1) and (1∶2) were overmatch the groups that decocted individual.Conclusion The compatibility and synergy of Codonopsis and Astragalus can enhance their immunoregulatory activity.关键词
党参/黄芪/煎煮形式/配伍比例/免疫调节Key words
Codonopsis pilosula/Astragalus membranaceus/decocted modality/compatibility proportion of medicines/immunoregulation分类
医药卫生引用本文复制引用
邵晶,杜丽东,孙政华,郭玫,吴国泰..煎煮形式及配伍比例对党参黄芪复方免疫调节作用的影响[J].中国临床药理学杂志,2017,33(10):917-920,938,5.基金项目
甘肃省教育厅科研基金资助项目(2013A-085) （2013A-085）
甘肃省高等学校基本科研基金资助项目(2013-1) （2013-1）
