中国临床药理学杂志2017，Vol.33Issue(10)：933-935,3.DOI:10.13699/j.cnki.1001-6821.2017.10.018
西罗莫司缓释微球的制备及其对淋巴细胞增殖的抑制作用研究
Preparation and inhibitory effect of sirolimus sustained-release microsphere on lymphocyte proliferation
刘炜 1王晓彤 1杨蕾 1王建华2
作者信息
- 1. 首都医科大学附属北京世纪坛医院药剂科,北京100038
- 2. 解放军总医院老年医学研究所,北京100853
- 折叠
摘要
Abstract
Objective To study the preparation and inhibitory effect of Sirolimus sustained-release microsphere on lymphocyte proliferation.Methods The sirolimus sustained-release microspheres were prepared by the emulsion solvent evaporation.The morphology of microspheres were observed by transmission electron microscope.The inhibitory rate of sirolimus sustained-release microspheres on human lymphocyte were detected by MTT assay.Results The average particle size of the microspheres was (186.73 ± 12.62) μm,drug loading was (28.74 ± 2.63)％,encapsulation efficiency was (73.53 ± 7.12)％.Sirolimus sustained-release microspheres can stably release in vitro,the cumulative release rate of 24 h was 47.3％,the cumulative release rate of 168 h was 93.15％.After the culture of 12,24,48,72,96 h,the inhibition rate of proliferation of the microspheres was 18.28％,33.96％,50.24％,73.91％,83.02％.Conclusion Sirolimus sustained-release microspheres had good sustained-release effect in vitro and inhibition of lymphocyte proliferation,providing the basis for the new formulation of sirolimus.关键词
西罗莫司/缓释微球制备/淋巴细胞增殖Key words
sirolimus/sustained-release microsphere/lymphocyte proliferation分类
医药卫生引用本文复制引用
刘炜,王晓彤,杨蕾,王建华..西罗莫司缓释微球的制备及其对淋巴细胞增殖的抑制作用研究[J].中国临床药理学杂志,2017,33(10):933-935,3.
