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西藏不同地区黑木耳多样性分析

施树 蒋厚阳 罗章

中国林副特产Issue(3):11-14,4.
中国林副特产Issue(3):11-14,4.DOI:10.13268/j.cnki.fbsic.2017.03.004

西藏不同地区黑木耳多样性分析

Analysis of Genetic Diversity and Relationship of Black Fungus from Different Regions of Tibet

施树 1蒋厚阳 2罗章3

作者信息

  • 1. 重庆旅游职业学院,重庆409000
  • 2. 西南大学食品科学学院,重庆北碚400700
  • 3. 西藏大学农牧学院,西藏林芝860000
  • 折叠

摘要

Abstract

Analysis genetic diversity and relationship of black fungus from different regions of Tibet by method of esterase isozyme enzyme spectrum and ISSR molecular markers.Using acrylamide protein electrophoresis technology on esterase isozyme of wild black fungus for electrophoresis;take a random amplificatioon of wild fungus by PCR-ISSR molecular marker techonlogy.Conduct product electrophoresis in agarose gel.Clustering and inheritance were analyzed by Ntsys 2.10 and PopGen32 software.Result shows: Samples of Lulang and Chayu have a closer relationship among wild fungus, there is a high genetic differentiation index among samples.This study provides a preliminary basis for species classification and hybrid of wild fungus of Tibet.

关键词

西藏/野生黑木耳/酯酶同工酶/ISSR

Key words

Tibet/Black fungus/Esterase isozyme/ISSR

分类

农业科技

引用本文复制引用

施树,蒋厚阳,罗章..西藏不同地区黑木耳多样性分析[J].中国林副特产,2017,(3):11-14,4.

基金项目

西藏自治区自然基金资助项目 ()

中国林副特产

1001-6902

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