中国农业科学2017，Vol.50Issue(8)：1370-1377,8.DOI:10.3864/j.issn.0578-1752.2017.08.002
小麦TaLox-B等位变异对脂肪氧化酶活性和面粉色泽的影响
Effects of TaLox-B Alleles on Lipoxygenase Activity and Flour Color in Wheats
摘要
Abstract
[Objective] Lipoxygenase (Lox) is closely related to flour color.The objective of this study was to investigate the influence of different allelic variations at TaLox-B loci on Lox activity and flour color in wheat to provide a theoretical basis for improving flour color and related quality traits in breeding programs.[Method] A total of 122 wheat cultivars and advanced lines from Henan province were used to measure the Lox activities and flour color by spectrophotometer and colorimeter.The functional markers Lox 16,Lox 18 and Lox-B23 were used to identify the polymorphism of the Lox genes on chromosome 4BS in current Henan wheat cultivars and advanced lines.[Result] There were significant differences of Lox activities,L*,a* and b*values as well as Wht data among different cultivars.Genotyping results indicated two alleles TaLox-B1a and TaLox-B1b at TaLox-B1 locus in these wheat varieties,with 63.9％ and 39.1％,respectively.At TaLox-B2 locus,two alleles TaLox-B2a and TaLox-B2b were identified by the functional marker Lox-B23,with 57.4％ and 42.6％,respectively.It was also found two alleles TaLox-B3a and TaLox-B3b at the TaLox-B3 locus,with 41.8％ and 48.2％,respectively.Six allele combinations of Lox genes,i.e.,TaLox-B1a/TaLox-B2a/TaLox-B3a,TaLox-B1 a/TaLox-B2a/TaLox-B3b,TaLox-B1 a/TaLox-B2b/TaLox-B3b,TaLox-B1 b/TaLox-B2a/TaLox-B3a,TaLox-B1 b/TaLox-B2a/TaLox-B3b and TaLox-B1b/TaLox-B2b/TaLox-B3b were found with 41.8％,15.6％,6.6％,28.7％,5.7％ and 1.6％,respectively.Analysis of association of Lox alleles at different TaLox-B loci and Lox activities and flour color parameters indicated that the allelic variation at a single locus showed diverse effects on Lox activities and flour color.For a* value,the allelic effect was not significant.For Lox activities,the order of effect was TaLox-B2a ＞ TaLox-B2b (P＜0.05),TaLox-B3a ＞ TaLox-B3b (P＜ 0.01).The Wht value of TaLox-B2a was significantly lower than that of TaLox-B2b (P＜0.01),and TaLox-B3a showed also a significant lower Wht value than TaLox-B3b (P＜0.05).These results suggest that TaLox-B2 and TaLox-B3 significantly affect Lox activities and flour color.Further analysis found that the genotype TaLox-B1a/TaLox-B2a/TaLox-B3a had the highest Lox activity and b* and the lowest a* and Wht value,and TaLox-B1b/TaLox-B2b/TaLox-B3b had the lowest Lox activity and b* and the highest L*,a* and b* values.[Conclusion] Among the 6 different combinations of three Lox genes in Henan wheat varieties,the genotype TaLox-B1a/TaLox-B2a/TaLox-B3a possess the highest Lox activity and the lowest Wht value (P＜0.05),and wheat cultivars with TaLox-B1b/TaLox-B2b/TaLox-B3b have the lowest Lox activity and the highest Wht value (P＜0.05).Therefore,the genetic control of Lox activities plays a key role in the improvement of wheat quality.关键词
普通小麦/脂肪氧化酶基因/功能标记/面粉色泽/河南Key words
common wheat/lipoxygenase gene/functional marker/flour color/Henan引用本文复制引用
张福彦,陈锋,程仲杰,杨保安,范家霖,陈晓杰,张建伟,陈云堂,崔龙..小麦TaLox-B等位变异对脂肪氧化酶活性和面粉色泽的影响[J].中国农业科学,2017,50(8):1370-1377,8.基金项目
河南省基础与前沿技术研究计划(162300410169)、河南省科技攻关计划(172102110069)、河南省小麦产业技术体系建设专项资金(Z2010-01-04) （162300410169）
