中国全科医学2017，Vol.20Issue(16)：1968-1972,5.DOI:10.3969/j.issn.1007-9572.2017.16.013
不同肠道病毒感染手足口病患儿儿茶酚胺、S-100蛋白及D-乳酸水平变化及其临床意义
Changing Level of Catecholamine,S-100 Protein and D-lactic Acid of Children with Hand-foot-and-mouth Disease Infected by Different Enterovirus and Its Clinical Significance
摘要
Abstract
Objective To investigate the changing level of catecholamine(CA),S-100 protein and D-lactic acid of children with hand-foot-and-mouth disease(HFMD) infected by different enterovirus and its clinical significance.Methods A total of 129 children with HFMD hospitalized in Kunming Children′s Hospital from April to July 2014 were enrolled.With GC-2016γ radioimmunoassay counter,CA 〔including noradrenaline(NE),adrenaline(AD) and dopamine(DA)〕 was measured by radioimmunoassay;with the American Boxlight ELx800NB microplate reader,the S-100 protein was measured by enzyme-linked immunosorbent assay;and D-lactic acid was measured by enzyme-linked immunosorbent assay.With 7500PCR amplifier produced by ABI company in Germany,the real-time quantitative PCR method was used to detect and classify fecal pathogens.Results Of the 129 children with HFMD,57(44.2%) were enterovirus 71(EV71)-positive,26(20.2%) were 16-type coxsackievirus A(CoxA16)-positive,the detection of 20(15.5%) was positive in other enteroviruses,and the detection of 26(20.1%) was negative in enteroviruses.There were no significant differences in NE,AD,DA and S-100 protein levels among children with HFMD infected by different enterovirus(P>0.05).The levels of D-lactic acid in the subjects were significantly different(P<0.05).The level of D-lactic acid in HFMD children of EV71-positive was higher than that in children of other enteroviruses-positive and enteroviruses-negative.Conclusion There are no differences in levels of CA and S-100 protein among children with HFMD infected by different enterovirus.The level of D-lactic acid in children with HFMD by EV71 is higher and the permeability of intestinal barrier is increased.关键词
儿童/手足口病/肠道病毒感染/儿茶酚胺类/S100蛋白质类/D-乳酸Key words
Child/Hand/foot and mouth disease/Enterovirus infections/Catecholamines/S100 proteins/D-lactic acid分类
医药卫生引用本文复制引用
王美芬,陈韬,顾涛,罗云娇,杜曾庆,王明英..不同肠道病毒感染手足口病患儿儿茶酚胺、S-100蛋白及D-乳酸水平变化及其临床意义[J].中国全科医学,2017,20(16):1968-1972,5.基金项目
云南省昆明市卫生人才培养项目及十百千工程项目(2016-SW省-53) （2016-SW省-53）
云南省昆明市科技计划重点项目(2015-02-S-01226) （2015-02-S-01226）
