中国全科医学2017，Vol.20Issue(16)：1978-1982,5.DOI:10.3969/j.issn.1007-9572.2017.16.015
青海地区不同海拔藏汉民族子痫前期患者人类白细胞抗原G表达研究
Expression of Human Leucocyte Antigen G in Tibetan and Han Patients with Preeclampsia at Different Altitudes of Qinghai Area
摘要
Abstract
Objective To investigate the expression of human leucocyte antigen G(HLA-G) in Tibetan and Han patients with preeclampsia at different altitudes of Qinghai Area.Methods 30 Han puerperas delivered in Affiliated Hospital of Qinghai University(an altitude of 2 200 m) from January 2015 to January 2016 were selected,and among them 16 with preeclampsia,14 of normal pregnancy;another 21 Tibetan puerperas delivered in Yushu Tibetan Autonomous Prefecture Hospital(an altitude of 3 700 m) during the same period were also selected,and among them 10 with preeclampsia,11 of normal pregnancy.The expression of HLA-G mRNA and protein in maternal placenta was detected by real-time quantitative polymerase chain reaction(RT-PCR) and western blotting.Results There were significant differences in expression of HLA-G mRNA and protein among puerperas in different situations(P<0.05).The expression of HLA-G mRNA and protein among Han puerperas with preeclampsia at low altitudes,Tibetan puerperas of normal pregnancy at high altitudes and Tibetan puerperas with preeclampsia at high altitudes was significantly lower than that of Han puerperas of normal pregnancy at low altitudes(P<0.05).The expression of HLA-G mRNA and protein of Tibetan puerperas with preeclampsia at high altitudes was significantly lower than that of Tibetan puerperas of normal pregnancy at high altitudes(P<0.05).The expression of HLA-G protein of Tibetan puerperas with preeclampsia at high altitudes was significantly lower than that of Han puerperas with preeclampsia at low altitudes(P<0.05),while there was no significant difference in the expression of HLA-G mRNA and protein between Han low-altitude puerperas with preeclampsia and Tibetan high-altitude puerperas of normal pregnancy(P>0.05).There was no significant difference in the expression of HLA-G mRNA between Han low-altitude puerperas with preeclampsia and Tibetan high-altitude puerperas with preeclampsia(P>0.05).Conclusion The chronic hypoxia environment in plateau may affect the expression of HLA-G gene.The differential expression of HLA-G in Tibetan and Han nationalities at different altitudes may play an important role in the pathogenetic process of preeclampsia in Qinghai plateau area,and is one of the causes of high incidence of preeclampsia in the plateau region.关键词
先兆子痫/HLA抗原/高海拔/藏族/汉族Key words
Pre-eclampsia/HLA antigens/Altitude/Zang nationality/Han nationality分类
医药卫生引用本文复制引用
谢莹莺,赵海宁,裴青青,曲新霞,张振平,赵得雄..青海地区不同海拔藏汉民族子痫前期患者人类白细胞抗原G表达研究[J].中国全科医学,2017,20(16):1978-1982,5.基金项目
青海省(应用)基础研究计划项目(2013-Z-736) （应用）
