摘要
Abstract
Objective To investigate the lymphocyte subset and immunoglobulin features of peripheral blood in children with Epstein-Barr virus(EBV)-associated infectious mononucleosis(IM) and infected with chronic active EBV (CAEBV).Methods Thirty-four children infected with EBV who received diagnosis and treatment in Department of Pediatrics,the First Affiliated Hospital of Zhengzhou University from May 2010 to May 2016 were selected,and they had underwent the detection of lymphocyte subset and immunoglobulin in peripheral blood.Among them,20 cases in IM group and 14 cases in CAEBV group;20 healthy children after physical examination at the same period were also included as control group.Flow cytometry was used to detect CD+3 T lymphocytes,CD+4 T lymphocytes,CD+8 T lymphocytes,B lymphocytes,and natural killer cell count,and immunoglobulin IgA,IgG and IgM levels were measured by immunological scatting turbidity method.Results There were significant difference in white blood cell count,lymphocyte count,CD+3 T lymphocyte count,CD+4 T lymphocyte count,CD+8 T lymphocyte count,B lymphocyte count,natural killer cell count of peripheral blood among the three groups (P<0.05).The leukocyte count,lymphocyte count,CD+3 T lymphocyte count and CD+8 T lymphocyte count in peripheral blood in IM group were higher than those in control group,and the B lymphocyte count was lower than that in control group (P<0.05).Lymphocyte count,CD+3 T lymphocyte count,CD+4 T lymphocyte count,CD+8 T lymphocyte count,B lymphocyte count and natural killer cell count in peripheral blood in CAEBV group were lower than those in control group and IM group (P<0.05).The leukocyte count in peripheral blood in CAEBV group was lower than that in IM group (P<0.05).There were significant differences in IgA and IgG among the three groups (P<0.05).IgA and IgG in IM group and CAEBV group were higher than those in control group (P<0.05).Conclusion The CD+8 T lymphocyte proliferation of children with IM is the main reason of significant increasing of lymphocyte count,lymphocyte of children CAEBV reduces,with reduced T lymphocytes,B lymphocytes,and natural killer cell subsets as the main features.Immunoglobulin IgA and IgG are significantly increased in children with IM and CAEBV.Lymphocyte subsets detection contributes to the diagnosis and differential diagnosis of IM and CAEBV.关键词
传染性单核细胞增多症/疱疹病毒4型,人/T淋巴细胞亚群/免疫球蛋白同型Key words
Infectious mononucleosis/Herpesvirus 4/human/T-lymphocyte subsets/Immunoglobulin isotypes分类
医药卫生
