中国输血杂志2017，Vol.30Issue(4)：398-401,4.DOI:10.13303/j.cjbt.issn.1004-549x.2017.04.024
血清学检测柳州地区RhD初筛阴性献血者D变异体结果分析
Result analysis of D variants detected by serology method among preliminary screening RhD negative blood donors in Liuzhou area
李雄英 1李聚林 1许建荣 1孔庆芳 1谭庆芬 1杨珊 1徐阳曦1
作者信息
摘要
Abstract
Objective To study the distribution of D variants among preliminary screening RhD negative blood donors in Liuzhou.Methods 425 blood donors were preliminarily screened by saline method as RhD negative in Liuzhou.These specimens were confirmed as weak D by indirect antiglobulin test.Indirect antiglobulin test negative samples were confnmed as Del and real RhD negative by absorption and elution test.Monoclonal IgM anti-C,c,E,e reagents were used to identify Rh phenotype through saline method.Rh gene frequency,haplotype frequency and Rh phenotype desired value of RhD negative blood donors were calculated by reference found in literature.Hardy-Weinberg goodness of fit was tested.Results Among 425 preliminary screening RhD negative blood donors,99 (23.29％) D variants were identified in all,including 18 (4.24％) weak D and 81(19.05％) Del;326(76.71％) were real RhD negative.The distribution of Rh phenotype was as followed:18 weak D:Ccee 9 (50.00％) ＞CCee4 (22.22％) ＞ccEe 3 (16.67％) ＞CCEe 1 (5.56％) =ccee 1 (5.56％);81 Del:Ccee 47 (58.03％) ＞CCee 17 (20.99％) ＞ccee 11 (13.58％) ＞ccEe 5 (6.17％) ＞ccEE 1 (1.23％);326 real RhD negative:ccee 199(61.04％) ＞Ccee 83(25.46％) ＞CCee 22(6.75％) ＞ccEe 16(4.91％) ＞ccEE 3 (0.92％) ＞CcEe 2(0.61％) ＞CCEe 1 (0.31％).The observed value and the desired value of Rh phenotype were not statistically significant (P＞0.05).The data was analyzed well based on Hardy-Weinberg law.The characteristic of haplotype frequency was cde (0.696 7) ＞ Cde(0.261 6) ＞ cdE(0.041 7) ＞ CdE(0.000 0).The characteristic of gene frequency was c(0.739 5)＞C(0.2605),e (0.960 7) ＞E (0.039 3).Conclusion The detection rate of D variants among preliminary screening RhD negative blood donors in Liuzhou is high,so determining positive weak D and Del blood donors can improve transfusion safety.关键词
RhD阴性/献血者/弱D/Del型/Rh表型Key words
RhD negative/volunteer donor/weak D/Del/Rh phenotype分类
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李雄英,李聚林,许建荣,孔庆芳,谭庆芬,杨珊,徐阳曦..血清学检测柳州地区RhD初筛阴性献血者D变异体结果分析[J].中国输血杂志,2017,30(4):398-401,4.
