中国实用口腔科杂志2017，Vol.10Issue(5)：291-293,297,4.DOI:10.19538/j.kq.2017.05.009
侵袭性牙周炎龈沟液中牙龈卟啉单胞菌与齿垢密螺旋体对有机酸影响研究
Influence of Porphyromonas gingivalis and Treponema denticola in gingival crevicular fluid of patients with aggressive periodontitis on organic acid
摘要
Abstract
Objective To investigate the influence of Porphyromonas gingivalis (Pg) and Treponema denticola (Td) in gingival crevicular fluid (GCF) of patients with aggressive periodantitis (AgP) on organic acid.Methods Forty cases of AgP and 40 healthy volunteers in our hospital were selected and GCF was collected.GCF in booth of same quadrant of patients was collected,and plaque index (PLI),sampling location,probing depth (PD),sulcus beeding index (SBI) and attachment loss (AL) were recorded.CE was used to detect the concentration of organic acid;PCR was used to detect Pg and Td.The gray value of amplification entry stripe,which was detected positive,was given quantitative analysis;amplification target bars and brightness ratio showed the relative volune of target gene,which was PCR product quantity.Resuits PLI,PD,AL,concentration of acid,Pg/Td detection rate and Pg/Td product quantity of AgP group were higher than those of healthy group (P ＜ 0.05);Pg butyric acid concentration at positive detection point of AgP group was higher than that at negative detection point (P ＜ 0.05);organic acid concentration at Td positive detection point was higher than that at negative group(P ＜ 0.05).Conclusion Organic acid concentration in GCF of AgP patients shows product quantity of Pg and Td,which plays a certain guiding role in organic acid concentration of AgP.关键词
侵袭性牙周炎/牙龈卟啉单胞菌/齿垢密螺旋体/有机酸Key words
aggressive periodontitis, AgP/Porphyromonas gingivalis, Pg/Treponema denticola, Td/organic acid分类
医药卫生引用本文复制引用
苏娟娟,薛鹏..侵袭性牙周炎龈沟液中牙龈卟啉单胞菌与齿垢密螺旋体对有机酸影响研究[J].中国实用口腔科杂志,2017,10(5):291-293,297,4.基金项目
国家自然科学基金(81100759) （81100759）
