中国听力语言康复科学杂志2017，Vol.15Issue(3)：181-184,4.DOI:10.3969/j.issn.1672-4933.2017.03.006
低剂量COCl2预处理降低耳蜗毛细胞和螺旋神经元的顺铂毒性
The Protection of Low Doses of CoCl2 Pretreatment in Cochlear Hair Cells and Spiral Ganglions Injured by Cisplatin
摘要
Abstract
Objective To sduty the protection of low doses of CoCl2 pretreatment in cochlear hair cells and spiral ganglions which are injured by cisplatin.Methods We culture the cochlear basement membrane in vitro and divide them into four groups randomly:normal group,100uM CoCl2 treatment group,200μM cisplatin treatment group,100μM CoCl2 and pretreatment plus 200uM cisplatin intervention group.We observe the cell status and count with immunofluorescence staining,detect the expression quantity of Caspase 3,HIF-1αt and NF-kB with Western blot.Results The CoCl2 treatment group is the same as the normal group,While the cisplatin treatment group has extremely injured hair cells and spiral ganglions compared to the normal group.The survival cell number of hair cell and spiral ganglions in CoCl2 pretreatment group is 2.81,1.4 times as many as the cisplatin treatment group.The western blot results indicate the expression quantity of CoCl2 pretreatment group and the cisplatin treatment group is 2.5,3.91 times as many as the normal group.Conclusion CoCl2 pretreatment can reduce the oxidative stress-mediated injury caused by cisplatin on hair cells and spiral ganglions.关键词
氯化钴/顺铂耳毒性/毛细胞/螺旋神经节Key words
CoCl2/Cisplatin induced oto toxicity/Hair cells/Spiral ganglions引用本文复制引用
李浩楠,王东霞,王泺璎,范东艳,王苹,于姝媛..低剂量COCl2预处理降低耳蜗毛细胞和螺旋神经元的顺铂毒性[J].中国听力语言康复科学杂志,2017,15(3):181-184,4.基金项目
国家自然科学基金地区科学基金项目(81260422) （81260422）
吉林省自然科学基金资助项目(20150101171J) （20150101171J）
