中国现代医学杂志2017，Vol.27Issue(11)：1-7,7.DOI:10.3969/j.issn.1005-8982.2017.11.001
LRIG1在卵巢浆液性囊腺癌细胞对依托泊苷敏感性中的作用研究
Role and mechanism of LRIG1 in SKOV3, siRNALRIG1 KOV3, KOV3/VP16 and siRNALRIG1 KOV3/VP16
摘要
Abstract
Objective To investigate the role and possible mechanism of LRIG1 in SKOV3, siRNALRIG1 KOV3, KOV3/VP16 and siRNALRIG1 KOV3/VP16. Methods The cell proliferation of SKOV3, siRNALRIG1 KOV3, KOV3/VP16 and siRNALRIG1 KOV3/VP16 were detected by MMT at different concentrations of VP16, which had been con-cultured for 48 hours. Cell proliferation was detected by plate cloning. Cell apoptosis was detected by flow cytometry. LRIG1 gene expression was detected by quantitative real-time PCR. Results IC50 of SKOV3, siRNALRIG1 KOV3, KOV3/VP16 and siRNALRIG1 KOV3/VP1 were 62.90 μg/L, 115.49 μg/L, 156.50μg/L and 195.42 μg/L, respectively, with the drug resistance index of 1.8, 2.5 and 3.1 respectively. Compared with SKOV3, the cloning rates had increased (the mean differences were 8.000, 37.692 and 72.000, P<0.05), the LRIG1 mRNA (the mean differences were 2.994, 88.366 and 279.004, P< 0.05) and apoptotic cells (the mean difference were 48.942, 63.48583.659, P < 0.05) had decreased significantly in siRNALRIG1 KOV3, SKOV3/VP16 and siRNALRIG1 KOV3/VP16 cells. Compared with siRNALRIG1 KOV3, the cloning rates had in-creased (the mean differences were 25.000 and 58.824, P<0.05), the LRIG1 mRNA (the mean differences were 47.478 and 180.147, P<0.05) and apoptotic cells (the mean differences were 53.678 and 42.687, P<0.05) had decreased in SKOV3/VP16 and siRNALRIG1 KOV3/VP16 cells (P< 0.05). Compared with SKOV3/VP16, the cloning rates had increased (the mean difference was 10.000, P<0.05), the LRIG1 mRNA (the mean difference was 92.784, P<0.05) and apoptotic cells (the mean difference was 37.625, P<0.05) had decreased in siRNA LRIG1 KOV3/VP16 cells (P<0.05). Conclusions The LRIG1 gene can affect drug sensitivity of SKOV3 for lower LRIG1 could decrease the apoptosis of cells.关键词
卵巢癌/耐药/亮氨酸重复序列免疫球蛋白样结构域基因-1Key words
ovarian cancer/drug resistance/leucine-rich repeats and immunoglobulin-like domains-1分类
生物科学引用本文复制引用
阳华,叶元,肖胜军,雷小妹,罗小红..LRIG1在卵巢浆液性囊腺癌细胞对依托泊苷敏感性中的作用研究[J].中国现代医学杂志,2017,27(11):1-7,7.基金项目
桂林市科学研究与技术开发计划项目(No:20130120-3) （No:20130120-3）
