中国现代医学杂志2017，Vol.27Issue(11)：14-19,6.DOI:10.3969/j.issn.1005-8982.2017.11.003
ABCG1在肿瘤坏死因子α诱导的氧化应激中的机制研究
ABCG1 inhibits TNF-α-induced oxidative stress by regulating NADPH oxidase and superoxide dismutase
摘要
Abstract
Objective To explore the role of ATP-binding cassette sub-family G member 1 (ABCG1) in oxidative stress production induced by tumor necrosis factor α (TNF-α) and its possible mechanisms. Methods Human umbilical vein endothelial cells (HUVECs) were transfected with specific ABCG1 siRNA or ABCG1 overexpression plasmid or pretreated with liver X receptor agonist T0901317, then were cultured with TNF-αfor 12 hours. Intracellular reactive oxygen species (ROS) levels were measured using 6-carboxy-2, 7-dichlorodihydrofluorescein diacetate, diacetoxymethyl ester (CDCFHDA-AM) fluorescence and nicotinamide ade-nine dinucleotide phosphate-oxidase (NADPH oxidase) activity was measured by spectrophotometer. Real time PCR and Western blot were employed to measure the expression of Nox4, one of NADPH oxidase subunit and the expression of superoxide dismutase (SOD). Results ABCG1 upregulation inhibited TNF-α-induced oxidative stress. Furthermore, the activity of NADPH oxidase and the expression of Nox4 were also suppressed by ABCG1 overexpression, but the expression of antioxidant SOD was promoted. Conversely, downregulation of ABCG1 by ABCG1 siRNA both increased the ROS production and promoted the NADPH oxidase activity and Nox4 expression. However, the expression of SOD1 was inhibited. Conclusions The results suggest thatABCG1 attenuates TNF-α-induced oxidative stress by regulating NADPH oxidase and SOD.关键词
三磷酸腺苷结合盒转运体G1/氧化应激/还原型烟酰胺腺嘌呤二核苷酸磷氧化酶/超氧化物歧化酶Key words
adenosine triphosphate binding cassette transporter G/oxidative stress/reduced nicotinamide adenine dinucleotide phosphate oxidase/superoxide dismutase分类
医药卫生引用本文复制引用
薛嘉虹,朱参战,胡艳超,栾春红..ABCG1在肿瘤坏死因子α诱导的氧化应激中的机制研究[J].中国现代医学杂志,2017,27(11):14-19,6.基金项目
国家自然科学基金(No:81100210) （No:81100210）
