中国现代医学杂志2017，Vol.27Issue(11)：20-25,6.DOI:10.3969/j.issn.1005-8982.2017.11.004
PPARγ1基因转染对心肌缺血再灌注后的影响和意义
Effect of myocardial PPARγ1 gene transfection in rat after myocardial ischemia reperfusion injury
摘要
Abstract
Objective To investigate the effects of overexpression of peroxisome proliferator-activated receptor γ1 (PPARγ1) gene in myocardial cells on the changes of hemodynamics, the myocardial infract areas and the concentrations of inserum cTnI and tissue MMP-9 in rats after myocardial ischemia-reperfusion injury. Methods Thirty SD rats were randomly divided into three groups (n=10): SHAM group, MIRI group and PPARγ1 group. In the SHAM group and the MIRI group, myocardial tissues were transfected with recom-binant adenovirus vector encoding enhanced green fluorescent protein (Ad-EGFP) via coronary artery. In PPARγ1 group, myocardial tissues were transfected with recombinant adenovirus vector mediated human PPARγ1 gene (Ad-PPARγ1). Three days after gene transfection, rats were operated for MIRI experiment. In group MIRI and PPARγ1, rats were subjected to 30 min of ischemia induced by ligating the left anterior descending branch (LADB) followed by 120 min of reperfusion. In the SHAM group, the rats only underwent open-chest surgery without ligation on the LADB. The HR, MAP, LVSP, LVEDP and (± dp/dt max) were observed at T0 (before ligation), T1 (ligation after 30 min), T2 (reperfusion after 30 min), T3 (reperfusion after 120 min). The myocardial infract areas, concentrations of cTnI in serum and MMP-9 in myocardial tissue were also assessed at 120 min after reperfusion. Results HR, LVSP, MAP (excepted at T3) and (± dp/dt max) were significantly reduced and LVEDP was increased in the MIRI group and PPARγ1 group compared to T0 (P<0.05). HR, LVSP, MAP (excepted at T3) and (± dp/dt max) in the MIRI group and PPARγ1 group were sig-nificantly decreased compared to the SHAM group (P<0.05). (± dp/dt max), LVSP at T2 and T3, MAP at T3 in the PPARγ1 group showed a significant increase compared with the MIRI group. There was no myocardial infarction in the SHAM group, and there was no significant difference in the myocardial ischemia area between MIRI group and PPARγ1 group (P>0.05). The myocardial ischemia areas and the concentration of cTnI in serum and MMP-9 in myocardial tissue in the MIRI group and PPARγ1 group were significantly higher than those of the SHAM group, meanwhile these results in PPARγ1 group were significantly less than the MIRI group (P<0.05). Conclusions Overexpression of PPARγ1 gene could protect myocardial by improving hemodynamic parameters, decreasing the infarct size and reducing the concentration of cTnI in serum and MMP-9 in tissue when myocardial ischemia-reperfusion injury happens.关键词
心肌缺血再灌注损伤/过氧化物酶体增殖物激活受体γ1/血流动力学/心肌梗死面积/心肌肌钙蛋白I/基质金属蛋白酶-9Key words
myocardial ischemia-reperfusion injury/peroxisome proliferator-activated receptor γ1/hemo-dynamic/myocardial infarction size/cardiac troponin I/myocardial matrix metalloproteinase-9分类
医药卫生引用本文复制引用
刘永平,魏来,陈文雁,孔高茵,刘景诗..PPARγ1基因转染对心肌缺血再灌注后的影响和意义[J].中国现代医学杂志,2017,27(11):20-25,6.基金项目
湖南省教育厅科研项目(No:16C0971) （No:16C0971）
