中国现代医学杂志2017，Vol.27Issue(11)：26-30,5.DOI:10.3969/j.issn.1005-8982.2017.11.005
西妥昔单抗联合铂类化疗对人喉鳞癌Hep-2细胞裸鼠移植瘤COX-2及MMP-7的影响
Impact of Cox-2 and MMP-7 of cetuximab combined with cisplatin on human laryngeal neoplasms Hep 2 cells nude mice transplanted tumor
摘要
Abstract
Objective To study effects of Cox-2 and MMP-7 of cetuximab combined with cisplatin on human laryngeal neoplasms Hep 2 cells nude mice transplanted tumor and its mechanism. Methods A total of 24 BALB/c human laryngeal neoplasms Hep 2 cells nude mice were established, and were divided into cetuximab group, cisplatin group, cetuximab combined cisplatin group, blank control group. The control group was treated with intraperitoneal injection 0.9% sodium chloride solution 0.2 ml, the cetuximab group with intraperitoneal injection 1,036μg/ml cetuximab injection, cisplatin group with intraperitoneal injection 3μg/ml cisplatin injection, and cetuximab combined with cisplatin group with intraperitoneal injection 1,036 μg/ml cetuximab injection and 3 μg/ml cisplatin injection. After four weeks, tumors growth, tumor tissue Cox-2 and MMP-7 protein expression and mRNA expression were compared. Results Volume and weight of tumors in the cetuximab group, cisplatin group, cetuximab combined cisplatin group were significantly lower than those in the control group (P<0.05). Volume and weight of tumors in the cetuximab combined cisplatin group were significantly low-er than those in cetuximab group, cisplatin group, inhibition rate were significantly higher cetuximab group, cis-platin group (P<0.05). Expression of COX-2, MMP-7 protein and mRNA in cetuximab group, cisplatin group, ce-tuximab combined cisplatin group were significanlty lower than those in the control group (P<0.05). Expressions of COX-2, MMP-7 protein and mRNA in cetuximab combined cisplatin group were significanlty lower than cetux-imab group, cisplatin group (P<0.05). Conclusions Cetuximab combination with cisplatin chemotherapy helps to inhibit laryngeal neoplasms Hep 2 cells nude mice transplanted tumor proliferation and growth. It may relat-ed to inhibiting tumor tissue Cox-2 and MMP-7 expression.关键词
西妥昔单抗/顺铂/喉癌/环氧化酶-2/基质金属蛋白酶-7Key words
cetuximab/cisplatin/laryngeal neoplasms/Cox-2/MMP-7分类
医药卫生引用本文复制引用
龚永谦,程爱兰,刘洁..西妥昔单抗联合铂类化疗对人喉鳞癌Hep-2细胞裸鼠移植瘤COX-2及MMP-7的影响[J].中国现代医学杂志,2017,27(11):26-30,5.基金项目
国家自然科学基金(No:81372894) （No:81372894）
湖南省教育厅科研项目(No:10C1136) （No:10C1136）
