中国现代医学杂志2017，Vol.27Issue(11)：36-40,5.DOI:10.3969/j.issn.1005-8982.2017.11.007
沉默中期因子对肝癌细胞恶性生物学行为的影响
Effect of midkine knockdown for malignant biological feature in human hepatocellular carcinoma
刘晶华 1邹玉 2吴钧 3常巍1
作者信息
- 1. 云南省第二人民医院 消化内科,云南 昆明 650001
- 2. 昆明医科大学统计教研室,云南 昆明 650000
- 3. 云南省昆明市第一人民医院 神经外科,云南 昆明 650031
- 折叠
摘要
Abstract
Objective To study the expression and roles of midkine (MDK) in human hepatocellular carcinoma (HCC). Methods A total of 50 HCC tissues and tumor adjacent tissues were collected to detect the expression of MDK by immunohistochemical staining. The expression of MDK in HepG2 cells was silenced by siRNA. Cell proliferation, apoptosis and invasion were detected by CCK-8, flow cytometry and Transwell, respectively. Results MDK expression was up-regulated in HCC tissues (P<0.05). Knockdown MDK in HCC cells was significantly down-regulated cell proliferation, invasion and induced apoptosis. Conclusions MDK is over-expressed in HCC tissues. Knockdown MDK can inhibit growth and invasion on HepG2 cells.关键词
中期因子/肝细胞癌/增殖/凋亡/侵袭Key words
MDK/hepatocellular carcinoma/proliferation/apoptosis/invasion分类
医药卫生引用本文复制引用
刘晶华,邹玉,吴钧,常巍..沉默中期因子对肝癌细胞恶性生物学行为的影响[J].中国现代医学杂志,2017,27(11):36-40,5.
