摘要
Abstract
Objective To study the expression of USP39, EZH2 and Sox17 in breast cancer tissue and its significance. Methods A total of 90 patients with breast cancer were divided into three groups according to different clinical features, 34 cases with carcinoma in situ, 41 cases with early invasive carcinoma and 15 cases with invasive carcinoma. RT-PCR was used to determine the differences in USP39, EZH2, Sox17, microRNA and autophagy gene expression in tumor tissue of three groups of patients. Results USP39 and EZH2 mRNA expression levels in tumor tissue of patients with invasive carcinoma were higher than those of patients with carcinoma in situ and early invasive carcinoma. Sox17 mRNA expression level was lower than those of patients with carcinoma in situ and early invasive carcinoma. MiR-29a and miR-149 expression levels were higher than those of patients with carcinoma in situ and early invasive carcinoma while miR-125b, miR-218 and miRNA-26a expression levels were lower than those of patients with carcinoma in situ and early invasive carcinoma. Autophagy gene LC3 mRNA expression level was higher than those of patients with carcinoma in situ and early invasive carcinoma while Beclin1, ATG2B and ATG4G mRNA expression levels were lower than those of patients with carcinoma in situ and early invasive carcinoma. USP39, EZH2 and Sox17 mRNA expression levels in tumor tissue of patients with breast cancer were directly correlated with the expression levels of related microRNA and autophagy genes. Conclusions USP39, EZH2 and Sox17 mRNA expression in tumor tissue of patients with breast cancer are closely related to the specific illness and prognosis, and are the marker genes of breast cancer.关键词
乳腺癌/USP39/EZH2/Sox17/自噬Key words
breast cancer/USP39/EZH2/Sox17/autophagy分类
医药卫生
